BRENDA - Enzyme Database show

Enzymic synthesis of steroid sulphates. XIII. Isolation and properties of dehydroepiandrosterone sulphotransferase from human foetal adrenals

Adams, J.B.; McDonald, D.; Biochim. Biophys. Acta 615, 275-278 (1980)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.8.2.15
cytosol
-
Homo sapiens
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.2.15
34000
-
2 * 34000, SDS-PAGE
Homo sapiens
2.8.2.15
68000
-
sucrose density gradient ultracentrifugation
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.2.15
3'-phosphoadenylylsulfate + dehydroepiandrosterone
Homo sapiens
3beta-hydroxyandrost-5-en-17-one
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
Homo sapiens
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.8.2.15
Homo sapiens
-
human
-
Purification (Commentary)
EC Number
Commentary
Organism
2.8.2.15
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.2.15
adrenal gland
foetal
Homo sapiens
-
2.8.2.15
fetus
foetal tissue is a much richer source of enzyme than adult
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.8.2.15
0.0201
-
-
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
2.8.2.15
-70°C, stored in glass ampoules , 0.05 M Tris-HCl buffer, pH 7.5, 0.1 mM DTT, 2% propylene glycol, 1 mg/ml protein, no significant difference in activity observed after storage for 3.5 months
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.15
3'-phosphoadenylylsulfate + 11-deoxycorticosterone
21-hydroxypregn-4-en-3,20-dione
645724
Homo sapiens
adenosine 3',5'-bisphosphate + 11-deoxycorticosterone sulfate
pregn-4-en-3-one 21-sulfate
645724
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + 17alpha-estradiol
estra-1,3,5(10)-triene-3,17alpha-diol
645724
Homo sapiens
adenosine 3',5'-bisphosphate + ?
-
-
-
r
2.8.2.15
3'-phosphoadenylylsulfate + 17beta-estradiol
estra-1,3,5(10)-triene-3,17alpha-diol
645724
Homo sapiens
adenosine 3',5'-bisphosphate + ?
-
-
-
r
2.8.2.15
3'-phosphoadenylylsulfate + 3alpha-hydroxy-5alpha-androstan-17-one
androsterone
645724
Homo sapiens
adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
androsterone sulfate
645724
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + 3beta-hydroxy-5alpha-androstan-17-one
epiandrosterone
645724
Homo sapiens
adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
-
645724
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + androst-5-ene-3beta,17beta-diol
-
645724
Homo sapiens
adenosine 3',5'-bisphosphate + ?
-
-
-
r
2.8.2.15
3'-phosphoadenylylsulfate + androst-5-ene-3beta17alpha-diol
-
645724
Homo sapiens
adenosine 3',5'-bisphosphate + ?
-
-
-
r
2.8.2.15
3'-phosphoadenylylsulfate + dehydroepiandrosterone
3beta-hydroxyandrost-5-en-17-one
645724
Homo sapiens
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
645724
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + epitestosterone
17alpha-hydroxyandrost-4-en-3-one
645724
Homo sapiens
adenosine 3',5'-bisphosphate + epitestosterone sulfate
androst-4-en-3-one 17-sulfate
645724
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + estrone
-
645724
Homo sapiens
adenosine 3',5'-bisphosphate + estrone sulfate
estra-1,3,5(10)-trien-17-one 3-sulfate
645724
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + etiocholanone
3alpha-hydroxy-5beta-androstan-17-one
645724
Homo sapiens
adenosine 3',5'-bisphosphate + etiocholanone sulfate
5beta-androstan-17-one 3-sulfate
645724
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + pregnenolone
3beta-hydroxypregn-5-en-20-one
645724
Homo sapiens
adenosine 3',5'-bisphosphate + pregnenolone sulfate
pregn-5-en-20-one 3-sulfate
645724
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + testosterone
17beta-hydroxyandrost-4-en-3-one
645724
Homo sapiens
adenosine 3',5'-bisphosphate + testosterone sulfate
androst-4-en-3-one 17-sulfate
645724
Homo sapiens
r
2.8.2.15
additional information
little or no activity with pregn-4-ene-11beta,21-diol-3,20-dione/cortisol and oestra-1,3,5(10)-triene-3,17beta-diol-3 methyl ether/17beta-oestradiol-3 methyl ether
645724
Homo sapiens
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.8.2.15
dimer
2 * 34000, SDS-PAGE
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.8.2.15
6.8
-
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.8.2.15
cytosol
-
Homo sapiens
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.2.15
34000
-
2 * 34000, SDS-PAGE
Homo sapiens
2.8.2.15
68000
-
sucrose density gradient ultracentrifugation
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.2.15
3'-phosphoadenylylsulfate + dehydroepiandrosterone
Homo sapiens
3beta-hydroxyandrost-5-en-17-one
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
Homo sapiens
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.8.2.15
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.2.15
adrenal gland
foetal
Homo sapiens
-
2.8.2.15
fetus
foetal tissue is a much richer source of enzyme than adult
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.8.2.15
0.0201
-
-
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.8.2.15
-70°C, stored in glass ampoules , 0.05 M Tris-HCl buffer, pH 7.5, 0.1 mM DTT, 2% propylene glycol, 1 mg/ml protein, no significant difference in activity observed after storage for 3.5 months
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.15
3'-phosphoadenylylsulfate + 11-deoxycorticosterone
21-hydroxypregn-4-en-3,20-dione
645724
Homo sapiens
adenosine 3',5'-bisphosphate + 11-deoxycorticosterone sulfate
pregn-4-en-3-one 21-sulfate
645724
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + 17alpha-estradiol
estra-1,3,5(10)-triene-3,17alpha-diol
645724
Homo sapiens
adenosine 3',5'-bisphosphate + ?
-
-
-
r
2.8.2.15
3'-phosphoadenylylsulfate + 17beta-estradiol
estra-1,3,5(10)-triene-3,17alpha-diol
645724
Homo sapiens
adenosine 3',5'-bisphosphate + ?
-
-
-
r
2.8.2.15
3'-phosphoadenylylsulfate + 3alpha-hydroxy-5alpha-androstan-17-one
androsterone
645724
Homo sapiens
adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
androsterone sulfate
645724
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + 3beta-hydroxy-5alpha-androstan-17-one
epiandrosterone
645724
Homo sapiens
adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
-
645724
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + androst-5-ene-3beta,17beta-diol
-
645724
Homo sapiens
adenosine 3',5'-bisphosphate + ?
-
-
-
r
2.8.2.15
3'-phosphoadenylylsulfate + androst-5-ene-3beta17alpha-diol
-
645724
Homo sapiens
adenosine 3',5'-bisphosphate + ?
-
-
-
r
2.8.2.15
3'-phosphoadenylylsulfate + dehydroepiandrosterone
3beta-hydroxyandrost-5-en-17-one
645724
Homo sapiens
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
645724
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + epitestosterone
17alpha-hydroxyandrost-4-en-3-one
645724
Homo sapiens
adenosine 3',5'-bisphosphate + epitestosterone sulfate
androst-4-en-3-one 17-sulfate
645724
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + estrone
-
645724
Homo sapiens
adenosine 3',5'-bisphosphate + estrone sulfate
estra-1,3,5(10)-trien-17-one 3-sulfate
645724
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + etiocholanone
3alpha-hydroxy-5beta-androstan-17-one
645724
Homo sapiens
adenosine 3',5'-bisphosphate + etiocholanone sulfate
5beta-androstan-17-one 3-sulfate
645724
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + pregnenolone
3beta-hydroxypregn-5-en-20-one
645724
Homo sapiens
adenosine 3',5'-bisphosphate + pregnenolone sulfate
pregn-5-en-20-one 3-sulfate
645724
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + testosterone
17beta-hydroxyandrost-4-en-3-one
645724
Homo sapiens
adenosine 3',5'-bisphosphate + testosterone sulfate
androst-4-en-3-one 17-sulfate
645724
Homo sapiens
r
2.8.2.15
additional information
little or no activity with pregn-4-ene-11beta,21-diol-3,20-dione/cortisol and oestra-1,3,5(10)-triene-3,17beta-diol-3 methyl ether/17beta-oestradiol-3 methyl ether
645724
Homo sapiens
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.8.2.15
dimer
2 * 34000, SDS-PAGE
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.8.2.15
6.8
-
-
Homo sapiens