Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dajani, R.; Hood, A.M.; Coughtrie, M.W.H.
    A single amino acid, Glu146, governs the substrate specificity of a human dopamine sulfotransferase, SULT1A3 (1998), Mol. Pharmacol., 54, 942-948.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.1 SULT1A1, DNA sequence determination and analysis, expression in Escherichia coli Homo sapiens
2.8.2.1 SULT1A3, wild-type and mutants, expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.8.2.1 E146A site-directed mutagenesis, reduced activity with dopamine and increased activity with 4-nitrophenol compared to wild-type SULT1A3, switch of specificity to wild-type SULT1A1 Homo sapiens
2.8.2.1 H143Y site-directed mutagenesis, reduced activity with dopamine compared to wild-type SULT1A3 Homo sapiens
2.8.2.1 H143Y/E146A site-directed mutagenesis, reduced activity with dopamine and increased activity with 4-nitrophenol compared to wild-type SULT1A3, switch of specificity to wild-type SULT1A1 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.1 0.002
-
 3'-phosphoadenylylsulfate SULT1A3 mutant H143Y, pH 7.4, 37°C Homo sapiens
2.8.2.1 0.0022
-
 dopamine wild-type SULT1A3, pH 7.4, 37°C Homo sapiens
2.8.2.1 0.003
-
 3'-phosphoadenylylsulfate SULT1A3 mutant E146A, pH 7.4, 37°C Homo sapiens
2.8.2.1 0.0038
-
 4-nitrophenol pH 7.4, 37°C Homo sapiens
2.8.2.1 0.0038
-
 4-nitrophenol wild-type SULT1A1 Homo sapiens
2.8.2.1 0.0043
-
 3'-phosphoadenylylsulfate wild-type SULT1A1, pH 7.4, 37°C Homo sapiens
2.8.2.1 0.0046
-
 3'-phosphoadenylylsulfate wild-type SULT1A3, pH 7.4, 37°C Homo sapiens
2.8.2.1 0.0048
-
 3'-phosphoadenylylsulfate SULT1A3 double mutant H143Y/E146A, pH 7.4, 37°C Homo sapiens
2.8.2.1 0.0056
-
 dopamine SULT1A3 mutant H143Y, pH 7.4, 37°C Homo sapiens
2.8.2.1 0.0061
-
 4-nitrophenol SULT1A3 mutant E146A, pH 7.4, 37°C Homo sapiens
2.8.2.1 0.0094
-
 4-nitrophenol SULT1A3 double mutant H143Y/E146A, pH 7.4, 37°C Homo sapiens
2.8.2.1 0.087
-
 dopamine SULT1A3 double mutant H143Y/E146A, pH 7.4, 37°C Homo sapiens
2.8.2.1 0.109
-
 dopamine wild-type SULT1A1, pH 7.4, 37°C Homo sapiens
2.8.2.1 0.127
-
 dopamine SULT1A3 mutant E146A, pH 7.4, 37°C Homo sapiens
2.8.2.1 1.38
-
 4-nitrophenol wild-type SULT1A3, pH 7.4, 37°C Homo sapiens
2.8.2.1 1.47
-
 4-nitrophenol SULT1A3 mutant H143Y, pH 7.4, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.1 Homo sapiens
-
 SULT1A3, is a M-PST enzyme form
-
2.8.2.1 Homo sapiens P50225 SULT1A1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.1 recombinant from Escherichia coli Homo sapiens
2.8.2.1 recombinant wild-type SULT1A3 and mutants from Escherichia coli Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.2.1 3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate Glu146 determines the substrate specificity Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.1 liver SULT1A1 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.1 3'-phosphoadenylyl sulfate + a phenol
-
 Homo sapiens adenosine 3',5'-bisphosphate + an aryl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 4-nitrophenol
-
 Homo sapiens adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + dopamine
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + dopamine SULT1A3 is specific for dopamine and other biogenic amines Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.1 additional information substrate specificity Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.2.1 SULT
-
 Homo sapiens