BRENDA - Enzyme Database show

Gene cloning, purification, and characterization of two cyanobacterial NifS homologs driving iron-sulfur cluster formation

Kato, S.; Mihara, H.; Kurihara, T.; Yoshimura, T.; Esaki, N.; Biosci. Biotechnol. Biochem. 64, 2412-2419 (2000)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.8.1.7
dithiothreitol
increase of enzyme activity at 50 mM
Synechocystis sp.
2.8.1.7
pyruvate
increase of enzyme activity at 5 mM
Synechocystis sp.
Cloned(Commentary)
EC Number
Commentary
Organism
2.8.1.7
-
Synechocystis sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.8.1.7
0.28
-
L-cysteine sulfinic acid
pH 7.5, 37°C, isoenzyme SsCsd2
Synechocystis sp.
2.8.1.7
0.43
-
L-selenocysteine
pH 7.5, 37°C, isoenzyme SsCsd1
Synechocystis sp.
2.8.1.7
0.59
-
L-selenocysteine
pH 7.5, 37°C, isoenzyme SsCsd2
Synechocystis sp.
2.8.1.7
17
-
L-cysteine sulfinic acid
pH 7.5, 37°C, isoenzyme SsCsd1
Synechocystis sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.8.1.7
Iron
[Fe2-S2]ferredoxin
Synechocystis sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.1.7
42000
-
2 * 42000, SDS-PAGE
Synechocystis sp.
2.8.1.7
87000
-
isoenzyme SsCsd1, gel filtration
Synechocystis sp.
2.8.1.7
88000
-
isoenzyme SsCsd2, gel filtration
Synechocystis sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.1.7
L-cysteine + [enzyme]-cysteine
Synechocystis sp.
-
L-alanine + [enzyme]-S-sulfanylcysteine
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.8.1.7
Synechocystis sp.
-
PCC6803
-
Purification (Commentary)
EC Number
Commentary
Organism
2.8.1.7
isoenzyme SsCsd1, isoenzyme SsCsd2 and isoenzyme SsCsd3
Synechocystis sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.8.1.7
4.5
-
isoenzyme SsCsd1, with L-cysteine sulfinic acid as substrate
Synechocystis sp.
2.8.1.7
4.8
-
isoenzyme SsCsd2, with L-cysteine sulfinic acid as substrate
Synechocystis sp.
2.8.1.7
5.8
-
isoenzyme SsCsd2, with L-selenocysteine as substrate
Synechocystis sp.
2.8.1.7
21
-
isoenzyme SsCsd1, with L-selenocysteine as substrate
Synechocystis sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.1.7
L-cysteine + [enzyme]-cysteine
-
645621
Synechocystis sp.
L-alanine + [enzyme]-S-sulfanylcysteine
-
-
-
?
2.8.1.7
L-cysteine + [enzyme]-cysteine
L-cysteine desulfuration requires a cysteine residue at the active site of the enzyme, but decomposition of L-selenocysteine and L-cysteine sufinic acid do not
645621
Synechocystis sp.
L-alanine + [enzyme]-S-sulfanylcysteine
-
-
-
?
2.8.1.7
L-cysteine sulfinic acid
L-cysteine desulfuration requires a cysteine residue at the active site of the enzyme, but decomposition of L-selenocysteine and L-cysteine sufinic acid do not
645621
Synechocystis sp.
L-alanine + sulfite
-
645621
Synechocystis sp.
?
2.8.1.7
L-cystine
-
645621
Synechocystis sp.
?
-
-
-
?
2.8.1.7
L-selenocysteine
L-cysteine desulfuration requires a cysteine residue at the active site of the enzyme, but decomposition of L-selenocysteine and L-cysteine sufinic acid do not
645621
Synechocystis sp.
L-alanine + selenium
-
-
-
?
2.8.1.7
L-selenocystine
-
645621
Synechocystis sp.
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.8.1.7
dimer
2 * 42000, SDS-PAGE
Synechocystis sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.8.1.7
37
-
assay at
Synechocystis sp.
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.8.1.7
3.2
-
L-cysteine sulfinic acid
pH 7.5, 37°C, isoenzyme SsCsd1
Synechocystis sp.
2.8.1.7
3.4
-
L-cysteine sulfinic acid
pH 7.5, 37°C, isoenzyme SsCsd2
Synechocystis sp.
2.8.1.7
4
-
L-selenocysteine
pH 7.5, 37°C, isoenzyme SsCsd2
Synechocystis sp.
2.8.1.7
15
-
L-selenocysteine
pH 7.5, 37°C, isoenzyme SsCsd1
Synechocystis sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.8.1.7
7
-
isoenzyme SsCsd1 with L-selenocysteine and isoenzyme SsCsd2 with L-selenocysteine and L-cysteine sulfinic acid as substrates
Synechocystis sp.
2.8.1.7
7.7
-
isoenzyme SsCsd1 with S-cysteine sulfinic acid as substrate
Synechocystis sp.
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.8.1.7
dithiothreitol
increase of enzyme activity at 50 mM
Synechocystis sp.
2.8.1.7
pyruvate
increase of enzyme activity at 5 mM
Synechocystis sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.8.1.7
-
Synechocystis sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.8.1.7
0.28
-
L-cysteine sulfinic acid
pH 7.5, 37°C, isoenzyme SsCsd2
Synechocystis sp.
2.8.1.7
0.43
-
L-selenocysteine
pH 7.5, 37°C, isoenzyme SsCsd1
Synechocystis sp.
2.8.1.7
0.59
-
L-selenocysteine
pH 7.5, 37°C, isoenzyme SsCsd2
Synechocystis sp.
2.8.1.7
17
-
L-cysteine sulfinic acid
pH 7.5, 37°C, isoenzyme SsCsd1
Synechocystis sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.8.1.7
Iron
[Fe2-S2]ferredoxin
Synechocystis sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.1.7
42000
-
2 * 42000, SDS-PAGE
Synechocystis sp.
2.8.1.7
87000
-
isoenzyme SsCsd1, gel filtration
Synechocystis sp.
2.8.1.7
88000
-
isoenzyme SsCsd2, gel filtration
Synechocystis sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.1.7
L-cysteine + [enzyme]-cysteine
Synechocystis sp.
-
L-alanine + [enzyme]-S-sulfanylcysteine
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.8.1.7
isoenzyme SsCsd1, isoenzyme SsCsd2 and isoenzyme SsCsd3
Synechocystis sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.8.1.7
4.5
-
isoenzyme SsCsd1, with L-cysteine sulfinic acid as substrate
Synechocystis sp.
2.8.1.7
4.8
-
isoenzyme SsCsd2, with L-cysteine sulfinic acid as substrate
Synechocystis sp.
2.8.1.7
5.8
-
isoenzyme SsCsd2, with L-selenocysteine as substrate
Synechocystis sp.
2.8.1.7
21
-
isoenzyme SsCsd1, with L-selenocysteine as substrate
Synechocystis sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.1.7
L-cysteine + [enzyme]-cysteine
-
645621
Synechocystis sp.
L-alanine + [enzyme]-S-sulfanylcysteine
-
-
-
?
2.8.1.7
L-cysteine + [enzyme]-cysteine
L-cysteine desulfuration requires a cysteine residue at the active site of the enzyme, but decomposition of L-selenocysteine and L-cysteine sufinic acid do not
645621
Synechocystis sp.
L-alanine + [enzyme]-S-sulfanylcysteine
-
-
-
?
2.8.1.7
L-cysteine sulfinic acid
L-cysteine desulfuration requires a cysteine residue at the active site of the enzyme, but decomposition of L-selenocysteine and L-cysteine sufinic acid do not
645621
Synechocystis sp.
L-alanine + sulfite
-
645621
Synechocystis sp.
?
2.8.1.7
L-cystine
-
645621
Synechocystis sp.
?
-
-
-
?
2.8.1.7
L-selenocysteine
L-cysteine desulfuration requires a cysteine residue at the active site of the enzyme, but decomposition of L-selenocysteine and L-cysteine sufinic acid do not
645621
Synechocystis sp.
L-alanine + selenium
-
-
-
?
2.8.1.7
L-selenocystine
-
645621
Synechocystis sp.
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.8.1.7
dimer
2 * 42000, SDS-PAGE
Synechocystis sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.8.1.7
37
-
assay at
Synechocystis sp.
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.8.1.7
3.2
-
L-cysteine sulfinic acid
pH 7.5, 37°C, isoenzyme SsCsd1
Synechocystis sp.
2.8.1.7
3.4
-
L-cysteine sulfinic acid
pH 7.5, 37°C, isoenzyme SsCsd2
Synechocystis sp.
2.8.1.7
4
-
L-selenocysteine
pH 7.5, 37°C, isoenzyme SsCsd2
Synechocystis sp.
2.8.1.7
15
-
L-selenocysteine
pH 7.5, 37°C, isoenzyme SsCsd1
Synechocystis sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.8.1.7
7
-
isoenzyme SsCsd1 with L-selenocysteine and isoenzyme SsCsd2 with L-selenocysteine and L-cysteine sulfinic acid as substrates
Synechocystis sp.
2.8.1.7
7.7
-
isoenzyme SsCsd1 with S-cysteine sulfinic acid as substrate
Synechocystis sp.