Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • McIver, L.; Baxter, R.L.; Campopiano, D.J.
    Identification of the [Fe-S] cluster-binding residues of Escherichia coli biotin synthase (2000), J. Biol. Chem., 275, 13888-13894.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.6 wild-type and mutants Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.8.1.6 C188S enzyme is insoluble Escherichia coli
2.8.1.6 C288T normal enzyme activity Escherichia coli
2.8.1.6 C53S no enzyme activity, spectrum shows no peak indicative of the presence of an [Fe-S] cluster Escherichia coli
2.8.1.6 C57S no enzyme activity, spectrum shows no peak indicative of the presence of an [Fe-S] cluster Escherichia coli
2.8.1.6 C60S no enzyme activity, spectrum shows no peak indicative of the presence of an [Fe-S] cluster Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.6 38650 39670 wild-type and mutant enzymes, electrospray mass spectrometry Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.6 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.6 wild-type and mutant enzymes Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.6 dethiobiotin + sulfur
-
 Escherichia coli biotin
-
 ?