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Literature summary extracted from

  • Lee, C.Y.; Hwang, J.H.; Lee, Y.S.; Cho, K.S.
    Purification and characterization of mouse liver rhodanese (1995), J. Biochem. Mol. Biol., 28, 170-176.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.1.1 Cd2+ more than 70% inhibition at 0.1 mM Mus musculus
2.8.1.1 Cu2+ more than 70% inhibition at 0.1 mM Mus musculus
2.8.1.1 Hg2+ more than 70% inhibition at 0.1 mM Mus musculus
2.8.1.1 Ni2+ more than 70% inhibition at 0.1 mM Mus musculus
2.8.1.1 Zn2+ more than 70% inhibition at 0.1 mM Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.1 8.3
-
 thiosulfate 25°C, pH 9.4 Mus musculus
2.8.1.1 12.5
-
 CN- 25°C, pH 9.4 Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.1 34000
-
 1 * 34000, SDS-PAGE Mus musculus
2.8.1.1 34800
-
 gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.1 thiosulfate + cyanide Mus musculus
-
 sulfite + thiocyanate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.1 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.1
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.1.1 liver
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.1.1 1071
-
 after Sephacryl S-200-HR gel filtration Mus musculus

Storage Stability

EC Number Storage Stability Organism
2.8.1.1 -70°C, 3 weeks, enzyme splits in two identical subunits with a molecular weight of 17000 Da Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.1 thiosulfate + cyanide
-
 Mus musculus sulfite + thiocyanate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.1.1 monomer 1 * 34000, SDS-PAGE Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.1.1 25
-
-
 Mus musculus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.8.1.1
-
 50
-
 Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.1.1 9.4
-
-
 Mus musculus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.8.1.1 Mus musculus isoelectric focusing in Phast Gel isoelectric focusing gel with pH range: 3 - 9
-
 6.6