BRENDA - Enzyme Database

Inhibition of rat liver rhodanese by di-, tricarboxylic, and alpha-keto acids

Oi, S.; J. Biochem. 78, 825-834 (1975)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.1.1
2-oxoglutarate
incubation of mitochondria with 2-oxoglutarate causes a significant decrease in activity
Rattus norvegicus
2.8.1.1
anions
inhibited by most anions at rather high concentration, the most active inhibitors are aromatic anions, incubation of mitochondria with sulfate and 2-oxoglutarate causes a significant decrease in activity
Rattus norvegicus
2.8.1.1
citrate
-
Rattus norvegicus
2.8.1.1
DL-isocitrate
-
Rattus norvegicus
2.8.1.1
fumarate
-
Rattus norvegicus
2.8.1.1
malate
-
Rattus norvegicus
2.8.1.1
oxaloacetate
-
Rattus norvegicus
2.8.1.1
pyruvate
-
Rattus norvegicus
2.8.1.1
SO42-
incubation of mitochondria with sulfate causes a significant decrease in activity
Rattus norvegicus
2.8.1.1
succinate
-
Rattus norvegicus
2.8.1.1
sulfite
-
Rattus norvegicus
2.8.1.1
thiocyanate
-
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.1.1
thiosulfate + cyanide
Rattus norvegicus
-
sulfite + thiocyanate
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.8.1.1
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.8.1.1
-
Rattus norvegicus
Reaction
EC Number
Reaction
Commentary
Organism
2.8.1.1
thiosulfate + cyanide = sulfite + thiocyanate
double displacement mechanism
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.1.1
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.8.1.1
600
-
main fraction of Sephadex G-200 gel chromatography
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.1.1
thiosulfate + cyanide
-
645511
Rattus norvegicus
sulfite + thiocyanate
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.8.1.1
More
purification yields two active fractions, of 17500 Da and 12600 Da by gel filtration
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.1.1
2-oxoglutarate
incubation of mitochondria with 2-oxoglutarate causes a significant decrease in activity
Rattus norvegicus
2.8.1.1
anions
inhibited by most anions at rather high concentration, the most active inhibitors are aromatic anions, incubation of mitochondria with sulfate and 2-oxoglutarate causes a significant decrease in activity
Rattus norvegicus
2.8.1.1
citrate
-
Rattus norvegicus
2.8.1.1
DL-isocitrate
-
Rattus norvegicus
2.8.1.1
fumarate
-
Rattus norvegicus
2.8.1.1
malate
-
Rattus norvegicus
2.8.1.1
oxaloacetate
-
Rattus norvegicus
2.8.1.1
pyruvate
-
Rattus norvegicus
2.8.1.1
SO42-
incubation of mitochondria with sulfate causes a significant decrease in activity
Rattus norvegicus
2.8.1.1
succinate
-
Rattus norvegicus
2.8.1.1
sulfite
-
Rattus norvegicus
2.8.1.1
thiocyanate
-
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.1.1
thiosulfate + cyanide
Rattus norvegicus
-
sulfite + thiocyanate
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.8.1.1
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.1.1
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.8.1.1
600
-
main fraction of Sephadex G-200 gel chromatography
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.1.1
thiosulfate + cyanide
-
645511
Rattus norvegicus
sulfite + thiocyanate
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.8.1.1
More
purification yields two active fractions, of 17500 Da and 12600 Da by gel filtration
Rattus norvegicus