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Literature summary extracted from

  • Low, S.C.; Harney, J.W.; Berry, M.J.
    Cloning and functional characterization of human selenophosphate synthetase, an essential component of selenoprotein synthesis (1995), J. Biol. Chem., 270, 21659-21664.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.9.3 gene selD, DNA sequence determination and analysis, subcloning and transient expression in enzyme deficient Escherichia coli resulting in poor complemetation of the bacteria by the human gene, and expression in mammalian HtTA cells together with human type I iodothyronine 5'-deiodinase and Xenopus tRNASeC Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.9.3 G269C site-directed mutagenesis, reduced activity, slightly reduced ATP binding Homo sapiens
2.7.9.3 G271R site-directed mutagenesis, reduced activity, slightly reduced ATP binding Homo sapiens
2.7.9.3 G274A site-directed mutagenesis, reduced activity and ATP binding Homo sapiens
2.7.9.3 G274D site-directed mutagenesis, reduced activity, no ATP binding Homo sapiens
2.7.9.3 G274V site-directed mutagenesis, no activity and ATP binding Homo sapiens
2.7.9.3 H275N site-directed mutagenesis, reduced activity, no ATP binding Homo sapiens
2.7.9.3 H275Y site-directed mutagenesis, increased catalytic activity and ATP binding Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.9.3 8-azido-ATP competitive to ATP Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.3 Homo sapiens P49903 gene selD
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.3 ATP + selenide + H2O
-
 Homo sapiens AMP + selenophosphate + phosphate
-
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