Literature summary extracted from

Cooper, R.A.; Kornberg, H.L.
Net formation of phosphoenolpyruvate from pyruvate by Escherichia coli (1965), Biochim. Biophys. Acta, 104, 618-620.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.7.9.2	F-	-	Escherichia coli
2.7.9.2	additional information	no inhibition by arsenate	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.9.2	Escherichia coli	-	strain Bm, a mutant of strain B devoid of phosphoenolpyruvate carboxylase	-

Storage Stability

EC Number	Storage Stability	Organism
2.7.9.2	unstable if stored in ice, but retains full activity for several days if stored at room temperature in the presence of EDTA and Mg2+	Escherichia coli

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.9.2	ATP + pyruvate + H2O	equilibrium lies far to the side of phosphoenolpyruvate formation	Escherichia coli	AMP + phosphoenolpyruvate + phosphate	-	r

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
2.7.9.2	22	-	retains full activity for several days if stored at room temperature in the presence of EDTA and Mg2+	Escherichia coli

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Release 2023.1 (January 2023)