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Literature summary extracted from

  • Sanchez, C.; Du, L.; Edwards, D.J.; Toney, M.D.; Shen, B.
    Cloning and characterization of a phosphopantetheinyl transferase from Streptomyces verticillus ATCC15003, the producer of the hybrid peptide-polyketide antitumor drug bleomycin (2001), Chem. Biol., 8, 725-738.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.8.7 medicine producer of the hybrid peptide-polyketide antitumor drug bleomycin Streptomyces verticillus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.7 svp gene cloned into pQE-70 and overproduced in Escherichia coli M15(pREP4) Streptomyces verticillus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.7 0.0031
-
 apo-[peptidyl carrier protein] pH 8.0, 37°C Streptomyces verticillus
2.7.8.7 0.0039
-
 apo-[peptidyl carrier protein] pH 8.0, 37°C Streptomyces verticillus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.7 26830
-
 calculated from amino acid sequence of His6-tagged svp Streptomyces verticillus
2.7.8.7 31000
-
 1 * 31000, SDS-PAGE Streptomyces verticillus
2.7.8.7 31600
-
 gel filtration Streptomyces verticillus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.7 CoA + apo-[acyl-carrier protein] Streptomyces verticillus
-
 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
2.7.8.7 CoA + apo-[acyl-carrier protein] Streptomyces verticillus ATCC 15003
-
 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.7 Streptomyces verticillus Q9F0Q6 strain ATCC15003, nucleotide sequence of the svp locus
-
2.7.8.7 Streptomyces verticillus ATCC 15003 Q9F0Q6 strain ATCC15003, nucleotide sequence of the svp locus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.7 recombinant enzyme, expressed in Escherichia coli Streptomyces verticillus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.7 CoA + apo-[acyl-carrier protein]
-
 Streptomyces verticillus adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
2.7.8.7 CoA + apo-[acyl-carrier protein]
-
 Streptomyces verticillus ATCC 15003 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
2.7.8.7 CoA + apo-[peptidyl carrier protein]
-
 Streptomyces verticillus adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein]
-
 r
2.7.8.7 CoA + apo-[peptidyl carrier protein]
-
 Streptomyces verticillus ATCC 15003 adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein]
-
 r
2.7.8.7 additional information posttranslational modification of carrier proteins, capable of modifying both type I and type II acyl carrier proteins and peptidyl carrier proteins, even form other Streptomyces sp. Streptomyces verticillus ?
-
 ?
2.7.8.7 additional information posttranslational modification of carrier proteins, capable of modifying both type I and type II acyl carrier proteins and peptidyl carrier proteins, even form other Streptomyces sp. Streptomyces verticillus ATCC 15003 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.8.7 monomer 1 * 31000, SDS-PAGE Streptomyces verticillus

Synonyms

EC Number Synonyms Comment Organism
2.7.8.7 PPTase
-
 Streptomyces verticillus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.8.7 0.183
-
 apo-[peptidyl carrier protein] pH 8.0, 37°C, substrate BlmI Streptomyces verticillus
2.7.8.7 1.43
-
 apo-[peptidyl carrier protein] pH 8.0, 37°C, substrate TcmM Streptomyces verticillus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.7 6.5 7
-
 Streptomyces verticillus