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Literature summary extracted from

  • Reitman, M.L.; Lang, L.; Kornfeld, S.
    UDP-N-acetylglucosamine: lysosomal enzyme N-acetylglucosamine-1-phosphotransferase (1984), Methods Enzymol., 107, 163-172.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.17 UDP
-
 Homo sapiens
2.7.8.17 UDP 5 mM, 63% inhibition Rattus norvegicus
2.7.8.17 UDPglucose competitive with respect to UDP-GlcNAc Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.17 0.038
-
 UDP-GlcNAc pH 6.5, 37°C Rattus norvegicus
2.7.8.17 183
-
 methyl-alpha-D-mannoside pH 6.5, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.17 Golgi apparatus
-
 Mus musculus 5794
-
2.7.8.17 Golgi apparatus
-
 Homo sapiens 5794
-
2.7.8.17 Golgi apparatus
-
 Rattus norvegicus 5794
-
2.7.8.17 Golgi membrane integral membrane protein Rattus norvegicus 139
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.17 Mg2+
-
 Homo sapiens
2.7.8.17 Mg2+ the divalent cation requirement is satisfied by Mn2+ better than by Mg2+ Rattus norvegicus
2.7.8.17 Mn2+
-
 Homo sapiens
2.7.8.17 Mn2+ the divalent cation requirement is satisfied by Mn2+ better than by Mg2+ Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.17 Homo sapiens
-
-
-
2.7.8.17 Mus musculus
-
-
-
2.7.8.17 Ovis aries
-
-
-
2.7.8.17 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.8.17 glycoprotein
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.8.17 brain
-
 Homo sapiens
-
2.7.8.17 BW5147 cell
-
 Mus musculus
-
2.7.8.17 fibroblast
-
 Homo sapiens
-
2.7.8.17 kidney
-
 Homo sapiens
-
2.7.8.17 liver
-
 Homo sapiens
-
2.7.8.17 liver
-
 Rattus norvegicus
-
2.7.8.17 liver
-
 Ovis aries
-
2.7.8.17 P388D1 cell
-
 Mus musculus
-
2.7.8.17 peripheral blood mononuclear cell
-
 Homo sapiens
-
2.7.8.17 placenta
-
 Homo sapiens
-
2.7.8.17 spleen
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.17 0.000733
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.8.17 0°C, 2 months, less than 10% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.17 additional information
-
 Homo sapiens ?
-
 ?
2.7.8.17 additional information UDPglucose is also a substrate with a catalytic efficiency about 12fold worse than UDP-GlcNAc. The enzyme phosphorylates lysosomal enzymes in an in vitro assay at least 100fold more efficiently than either other glycoproteins with similar carbohydrate chains or free oligosaccharides Rattus norvegicus ?
-
 ?
2.7.8.17 UDP-N-acetyl-D-glucosamine + methyl-alpha-D-mannoside
-
 Rattus norvegicus UMP + N-acetyl-D-glucosamine-phospho-(methyl-alpha-D-mannoside)
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.17 6.5 7.5 with Tris-HCl giving 50% more activity than sodium cacodylate Rattus norvegicus