BRENDA - Enzyme Database

Oligomerization of hamster UDP-GlcNAc:dolichol-P GlcNAc-1-P transferase, an enzyme with multiple transmembrane spans

Dan, N.; Lehrman, M.A.; J. Biol. Chem. 272, 14214-14219 (1997)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.7.8.15
R303K
catalytically inactive mutant enzyme, this mutant can be expressed in CHO-K1 cells and can bind tunicamycin efficiently, indicative of proper folding. The R303K mutant can inhibit exogenously expressed normal enzyme
Cricetulus griseus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.15
endoplasmic reticulum
an enzyme with multiple transmembrane spans
Cricetulus griseus
5783
-
2.7.8.15
membrane
enzyme with multiple transmembrane spans
Cricetulus griseus
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.8.15
34000
-
2 * 34000, the 67000 Da enzyme form is a homodimer of UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase or perhaps a hetero-oligomer with another microsomal component
Cricetulus griseus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.15
Cricetulus griseus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.15
CHO cell
-
Cricetulus griseus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645367
Cricetulus griseus
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645367
Cricetulus griseus
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.8.15
dimer
2 * 34000, the 67000 Da enzyme form is a homodimer of UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase or perhaps a hetero-oligomer with another microsomal component
Cricetulus griseus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.7.8.15
R303K
catalytically inactive mutant enzyme, this mutant can be expressed in CHO-K1 cells and can bind tunicamycin efficiently, indicative of proper folding. The R303K mutant can inhibit exogenously expressed normal enzyme
Cricetulus griseus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.15
endoplasmic reticulum
an enzyme with multiple transmembrane spans
Cricetulus griseus
5783
-
2.7.8.15
membrane
enzyme with multiple transmembrane spans
Cricetulus griseus
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.8.15
34000
-
2 * 34000, the 67000 Da enzyme form is a homodimer of UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase or perhaps a hetero-oligomer with another microsomal component
Cricetulus griseus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.15
CHO cell
-
Cricetulus griseus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645367
Cricetulus griseus
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645367
Cricetulus griseus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.8.15
dimer
2 * 34000, the 67000 Da enzyme form is a homodimer of UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase or perhaps a hetero-oligomer with another microsomal component
Cricetulus griseus