BRENDA - Enzyme Database

Role of the carboxyl terminus in stable expression of hamster UDP-GlcNAc:dolichol-P GlcNAc-1-P transferase

Zara, J.; Lehrman, M.A.; J. Biol. Chem. 269, 19108-19115 (1994)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.7.8.15
DELTA395-408
mutation fully eliminates enzyme expression in vivo
Cricetulus griseus
2.7.8.15
DELTA398-408
removal of the last 11 amino acids 398-408 from the enzyme has no significant effect on the catalytic activity, thermal stability, tunicamycin binding, reticular localization, or consumption of cellular dolichol phosphate
Cricetulus griseus
2.7.8.15
F395L/S396M/I397W
expression of the mutant enzyme
Cricetulus griseus
2.7.8.15
F395L/S396M/I397W/DELTA398-408
mutation fully eliminates enzyme expression in vivo
Cricetulus griseus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.15
endoplasmic reticulum
-
Cricetulus griseus
5783
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
Cricetulus griseus
the enzyme catalyzes the committed step for N-linked glycosylation
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.15
Cricetulus griseus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.15
CHO cell
-
Cricetulus griseus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645364
Cricetulus griseus
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645364
Cricetulus griseus
?
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
the enzyme catalyzes the committed step for N-linked glycosylation
645364
Cricetulus griseus
?
-
-
-
-
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.7.8.15
DELTA395-408
mutation fully eliminates enzyme expression in vivo
Cricetulus griseus
2.7.8.15
DELTA398-408
removal of the last 11 amino acids 398-408 from the enzyme has no significant effect on the catalytic activity, thermal stability, tunicamycin binding, reticular localization, or consumption of cellular dolichol phosphate
Cricetulus griseus
2.7.8.15
F395L/S396M/I397W
expression of the mutant enzyme
Cricetulus griseus
2.7.8.15
F395L/S396M/I397W/DELTA398-408
mutation fully eliminates enzyme expression in vivo
Cricetulus griseus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.15
endoplasmic reticulum
-
Cricetulus griseus
5783
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
Cricetulus griseus
the enzyme catalyzes the committed step for N-linked glycosylation
?
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.15
CHO cell
-
Cricetulus griseus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645364
Cricetulus griseus
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645364
Cricetulus griseus
?
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
the enzyme catalyzes the committed step for N-linked glycosylation
645364
Cricetulus griseus
?
-
-
-
-