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Literature summary extracted from

  • Kittelmann, M.; Klein, T.; Kragl, U.; Wandrey, C.; Ghisalba, O.
    CMP-N-acetyl neuraminic-acid synthetase from Escherichia coli: fermentative production and application for the preparative synthesis of CMP-neuraminic acid (1995), Appl. Microbiol. Biotechnol., 44, 59-67.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.7.43 synthesis the enzyme is used for preparative synthesis of CMP-N-acylneuraminate. Optimization of the culture conditions for the production of the enzyme and the detection of a colony variant of Escherichia coli K-235 that is an even better producer of the synthetase Escherichia coli

General Stability

EC Number General Stability Organism
2.7.7.43 stabilized against thermal inactivation by some salts, NaCl, Na2SO4 or Na2HPO4, or by glycerol Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.43 Escherichia coli
-
 K-235
-
2.7.7.43 Escherichia coli K-235
-
 K-235
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.43 partial Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.7.43 -20°C, partially purified enzyme can be stored in 43% glycerol or in 50 mM Tris/HCl or potassium phosphate, pH 7.5, for several months without loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.43 CTP + N-acylneuraminate
-
 Escherichia coli diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-acylneuraminate
-
 Escherichia coli K-235 diphosphate + CMP-N-acylneuraminate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.43 40
-
 50 min, complete inactivation without stabilizer, completely stable in presence of 1.0 M NaCl, 0.5 M Na2HPO4 or 25% v/v glycerol Escherichia coli
2.7.7.43 45
-
 10 min, complete deactivation Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.7.43 7
-
 most stable at neutral pH Escherichia coli