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Literature summary extracted from

  • Haverkamp, J.; Beau, J.M.; Schauer, R.
    Improved synthesis of CMP-sialates using enzymes from frog liver and equine submandibular gland (1979), Hoppe-Seyler's Z. Physiol. Chem., 360, 159-166.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.43 2-mercaptoethanol 20-30 mM, 3fold stimulation with N-acetylneuraminate as substrate, 1.5fold increase in activity with 4-O-methyl-N-acetylneuraminate as substrate Equus caballus
2.7.7.43 2-mercaptoethanol 1 mM is required for optimal activity of both crude and purified enzyme Pelophylax lessonae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.43 0.7
-
 N-acetylneuraminate pH 9, 37°C Equus caballus
2.7.7.43 1
-
 N-acetylneuraminate pH 9, 37°C Pelophylax lessonae
2.7.7.43 2.5
-
 4-O-methyl-N-acetylneuraminate pH 7, 37°C Equus caballus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.43 Equus caballus
-
-
-
2.7.7.43 Pelophylax lessonae
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.43 liver
-
 Pelophylax lessonae
-
2.7.7.43 submandibular gland
-
 Equus caballus
-

Storage Stability

EC Number Storage Stability Organism
2.7.7.43 -20°C, stable for at least 1 year Equus caballus
2.7.7.43 -20°C, stable for at least 1 year Pelophylax lessonae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.43 4-O-methyl-N-acetylneuraminate + CTP
-
 Equus caballus CMP-4-O-methyl-N-acetylneuraminate + diphosphate
-
 ?
2.7.7.43 4-O-methyl-N-acetylneuraminate + CTP no activity Pelophylax lessonae CMP-4-O-methyl-N-acetylneuraminate + diphosphate
-
 ?
2.7.7.43 CTP + N-acylneuraminate
-
 Equus caballus diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-acylneuraminate
-
 Pelophylax lessonae diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-glycolylneuraminate
-
 Equus caballus diphosphate + CMP-N-glycolylneuraminate
-
 ?
2.7.7.43 CTP + N-glycolylneuraminate
-
 Pelophylax lessonae diphosphate + CMP-N-glycolylneuraminate
-
 ?