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Literature summary extracted from

  • Seagrave, J.; Reyes, P.
    Pyrimidine nucleoside monophosphate kinase from rat bone marrow cells: chromatographic, electrophoretic, and sedimentation behavior of active and inactive enzyme forms (1986), Arch. Biochem. Biophys., 247, 76-83.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.4.14 dialysis against 20 mM phosphate, 1 mM MgCl2, 20% ethylene glycol, pH 8.0, 90% loss of activity Rattus norvegicus
2.7.4.14 freeze-thawing inactivates Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.14 DTNB 0.009 mM, 50% inhibition Rattus norvegicus
2.7.4.14 iodoacetate 0.05 mM, 50% inhibition Rattus norvegicus
2.7.4.14 NEM 0.035 mM, 50% inhibition Rattus norvegicus
2.7.4.14 p-hydroxymercuribenzoate 0.02 mM, 50% inhibition Rattus norvegicus
2.7.4.14 p-Hydroxymercuriphenyl sulfonate 0.02 mM, 50% inhibition Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.14 22500
-
 sucrose density gradient centrifugation Rattus norvegicus
2.7.4.14 26000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.14 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.14 bone marrow
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.7.4.14 -80°C, up to 12 months Rattus norvegicus
2.7.4.14 4°C, considerable loss of activity within 24 h, DTT stabilizes, more stable in 20 mM phosphate buffer, pH 8 than in Tris-HCl buffer Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.14 4
-
 24 h, in absence of dithiothreitol the purified enzyme shows considerable loss of activity Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.4.14 Rattus norvegicus isoelectric focusing 4.8 4.7