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Literature summary extracted from

  • Scott, E.M.; Wright, R.C.
    Kinetics and equilibria of pyrimidine nucleoside monophosphate kinase from human erythrocytes (1979), Biochim. Biophys. Acta, 571, 45-54.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.4.14 more stable in histidine than in phosphate buffer Homo sapiens
2.7.4.14 the purified enzyme is notably unstable Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.14 Mg2+ optimal concentration is 2-3 mM Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.14 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.14
-
 Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.4.14 ATP + UMP = ADP + UDP formation of a ternary complex, addition of substrates is random Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.14 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.14 337
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.7.4.14 -20°C, concentrated enzyme solution in 30% glycerol, less than 5% loss of activity per month Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.14 ATP + AMP
-
 Homo sapiens ADP + ADP
-
 r
2.7.4.14 ATP + CMP
-
 Homo sapiens ADP + CDP
-
 r
2.7.4.14 ATP + UMP
-
 Homo sapiens ADP + UDP
-
 r
2.7.4.14 dATP + UMP
-
 Homo sapiens dADP + ADP
-
 r
2.7.4.14 additional information formation of a ternary complex, addition of substrates is random Homo sapiens ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.14 20
-
 in absence of glycerol, the half-life of a preparation with a specific activity of 80 is about 10 min, inactivation is partially reversed by addition of 2-mercaptoethanol Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.4.14 additional information
-
 more stable in histidine buffer than in phosphate buffer Homo sapiens
2.7.4.14 6.5
-
 more stable than at higher pH Homo sapiens