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Literature summary extracted from

  • Suzuki, H.; Kaneko, T.; Ikeda, Y.
    Properties of polyphosphate kinase prepared from mycobacterium smegmatis (1972), Biochim. Biophys. Acta, 268, 381-390.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.1 ADP substrate inhibition Mycolicibacterium smegmatis
2.7.4.1 AMP 15% and 70% inhibition of the forward reaction at AMP/ADP ratios of 1 and 10, respectiverly Mycolicibacterium smegmatis
2.7.4.1 ATP substrate inhibition, at high concentrations Mycolicibacterium smegmatis
2.7.4.1 EDTA 10 mM, complete inhibition Mycolicibacterium smegmatis
2.7.4.1 F- 5 mM, 75% inhibition Mycolicibacterium smegmatis
2.7.4.1 Mg2+ 8 mM, complete inhibition Mycolicibacterium smegmatis
2.7.4.1 phosphate 5.5 mM and 11 mM, 33% and 49% inhibition of forward reaction Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.1 1
-
 Polyphosphate
-
 Mycolicibacterium smegmatis
2.7.4.1 1.1
-
 ADP pH 6.0, 37°C Mycolicibacterium smegmatis
2.7.4.1 2.5
-
 ATP pH 6.0, 37°C Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.1 Mg2+ required for activity Mycolicibacterium smegmatis
2.7.4.1 Mg2+ maximal activity at 2 mM Mycolicibacterium smegmatis
2.7.4.1 Mn2+ slightly Mycolicibacterium smegmatis
2.7.4.1 Mn2+ activation, can replace Mg2+ Mycolicibacterium smegmatis
2.7.4.1 additional information not activated by Ca2+ Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.1 Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.1 ammonium sulfate, Sephadex G-200 Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.1 0.18
-
-
 Mycolicibacterium smegmatis

Storage Stability

EC Number Storage Stability Organism
2.7.4.1 -20°C, 1 month, no loss of activity Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.1 ADP + (phosphate)n+1 substrates are polyphosphates or hexametaphosphate Mycolicibacterium smegmatis ATP + (phosphate)n
-
 r
2.7.4.1 ADP + (phosphate)n+1 no activity with GDP, diphosphate or tripolyphosphate Mycolicibacterium smegmatis ATP + (phosphate)n
-
 r
2.7.4.1 ATP + (phosphate)n
-
 Mycolicibacterium smegmatis ADP + (phosphate)n+1
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.1 37
-
 assay at Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.1 6.3 6.5
-
 Mycolicibacterium smegmatis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.1 5.8 7 approx. half-maximal activity at pH 5.8, approx. 85% of maximal activity at pH 7.0 Mycolicibacterium smegmatis