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Literature summary extracted from

  • Haas, M.J.; Dowding, J.E.
    Aminoglycoside-modifying enzymes (1975), Methods Enzymol., 43, 611-627.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.95 ammonium chloride or neomycin B stabilizes, E. coli JR39 enzyme Escherichia coli
2.7.1.95 dithiothreitol stabilizes and restores activity of denatured enzyme Staphylococcus aureus
2.7.1.95 dithiothreitol stabilizes and restores activity of denatured enzyme Escherichia coli
2.7.1.95 dithiothreitol stabilizes and restores activity of denatured enzyme Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.95 Gentamicin 1a with neomycin as substrate, no inhibition in E. coli strain JR66/W677 or Pseudomonas aeruginosa strain Ps49 Escherichia coli
2.7.1.95 Gentamicin 1a with neomycin as substrate, no inhibition in E. coli strain JR66/W677 or Pseudomonas aeruginosa strain Ps49 Pseudomonas aeruginosa
2.7.1.95 kanamycin substrate inhibition at 2 mM Staphylococcus aureus
2.7.1.95 tobramycin
-
 Escherichia coli
2.7.1.95 tobramycin
-
 Pseudomonas aeruginosa
2.7.1.95 tobramycin
-
 Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.95 0.3
-
 kanamycin pH 7.5, 35°C Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.95 Co2+ activation at 1 mM, can replace Mg2+ Pseudomonas aeruginosa
2.7.1.95 Mg2+
-
 Pseudomonas aeruginosa
2.7.1.95 Mg2+ required for activity Escherichia coli
2.7.1.95 Mn2+ activation at 1 mM, can replace Mg2+ Pseudomonas aeruginosa
2.7.1.95 Zn2+ activation at 1 mM, can replace Mg2+ Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.95 Escherichia coli
-
 strains JR35/W677, JR39/W677, JR66/W677, neomycin phosphotransferase II, ML1629, neomycin phosphotransferase I
-
2.7.1.95 Pseudomonas aeruginosa
-
 neomycin phosphotransferase II
-
2.7.1.95 Pseudomonas aeruginosa Ps49
-
 neomycin phosphotransferase II
-
2.7.1.95 Staphylococcus aureus
-
 multiple drug-resistant strain B294
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.95 ATP + kanamycin specific for ATP Staphylococcus aureus ADP + kanamycin 3'-phosphate
-
 ?
2.7.1.95 ATP + kanamycin specific for ATP Escherichia coli ADP + kanamycin 3'-phosphate
-
 ?
2.7.1.95 ATP + kanamycin specific for ATP Pseudomonas aeruginosa ADP + kanamycin 3'-phosphate
-
 ?
2.7.1.95 ATP + kanamycin specific for ATP Pseudomonas aeruginosa Ps49 ADP + kanamycin 3'-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.95 35 37 assay at Staphylococcus aureus
2.7.1.95 35 37 assay at Escherichia coli
2.7.1.95 35 37 assay at Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.95 45
-
 strain ML1629, 5 min, inactivation Escherichia coli
2.7.1.95 55
-
 strain H-9, 20 min stable Pseudomonas aeruginosa
2.7.1.95 65
-
 strain H-9, 5 min stable Pseudomonas aeruginosa
2.7.1.95 75
-
 strain H-9, inactivation Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.95 5.5 8 broad, no marked optimum Escherichia coli
2.7.1.95 5.5 8 broad, no marked optimum Pseudomonas aeruginosa
2.7.1.95 7 7.5
-
 Staphylococcus aureus
2.7.1.95 7.5
-
 with kanamycin Pseudomonas aeruginosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.95 7.5 9 stable Pseudomonas aeruginosa