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Literature summary extracted from

  • Waites, M.J.; Flanagan, T.
    Purification and characterization of D-xylulokinase from the pentose-fermenting yeast Pichia stipitis NCYC 1541 (1992), Enzyme Microb. Technol., 14, 975-979.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.17 EDTA no activation Scheffersomyces stipitis

General Stability

EC Number General Stability Organism
2.7.1.17 EDTA and MgCl2 partially stabilize Scheffersomyces stipitis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.17 EDTA weak Scheffersomyces stipitis
2.7.1.17 KCl weak Scheffersomyces stipitis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.17 0.28
-
 ATP
-
 Scheffersomyces stipitis
2.7.1.17 0.52
-
 D-xylulose
-
 Scheffersomyces stipitis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.17 Mg2+
-
 Scheffersomyces stipitis
2.7.1.17 Mn2+ 35% of the activation with Mg2+ Scheffersomyces stipitis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.17 71000
-
 2 * 71000, SDS-PAGE Scheffersomyces stipitis
2.7.1.17 120000 130000 gel filtration Scheffersomyces stipitis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.17 ATP + D-xylulose Scheffersomyces stipitis
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.17 Scheffersomyces stipitis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.17
-
 Scheffersomyces stipitis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.17 21.4
-
-
 Scheffersomyces stipitis

Storage Stability

EC Number Storage Stability Organism
2.7.1.17 -20°C, 1 month stable and 20% of original activity retained after 8 months Scheffersomyces stipitis
2.7.1.17 4°C, inactivation within 24 h Scheffersomyces stipitis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.17 ATP + D-xylulose
-
 Scheffersomyces stipitis ?
-
 ?
2.7.1.17 ATP + D-xylulose specific for D-isomer Scheffersomyces stipitis ADP + D-xylulose 5-phosphate
-
 r
2.7.1.17 ATP + L-erythrulose phosphorylation at 30% the rate of D-xylulose Scheffersomyces stipitis ADP + ?
-
 ?
2.7.1.17 GTP + D-xylulose no activity Scheffersomyces stipitis GDP + D-xylulose 5-phosphate
-
 ?
2.7.1.17 UTP + D-xylulose no activity Scheffersomyces stipitis UDP + D-xylulose 5-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.17 dimer 2 * 71000, SDS-PAGE Scheffersomyces stipitis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.17 30
-
-
 Scheffersomyces stipitis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.17 10 40 steady increase up to 30°C, rapid loss above, with 60% of maximal activity at 40°C Scheffersomyces stipitis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.17 7.2 7.8
-
 Scheffersomyces stipitis