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Literature summary extracted from

  • Müller, S.; Straub, A.; Lohse, M.J.
    Selectivity of beta-adrenergic receptor kinase 2 for G protein betagamma subunits (1997), FEBS Lett., 401, 25-29.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.15 G protein betagamma-subunit from bovine brain, beta-ARK 1 and 2: requirement, selectivity for betagamma subunits, both isoforms differentiate between defined betagamma subunits Bos taurus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.15 beta-ARK 1 and 2 are cloned and expressed in SF9 cells using the baculovirus expression system Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.15 propranolol beta-adrenergic antagonist, in presence no phosphorylation Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.15 Bos taurus
-
 enzyme exists in 2 subtypes: beta-ARK 1 and beta-ARK 2
-
2.7.11.15 Bos taurus
-
 beta-ARK 1 and 2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.15 recombinant beta-ARK 1 and 2 overexpressed in Sf9 cells Bos taurus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.15 additional information
-
 beta-ARK 2 has a 25% lower specific activity than beta-ARK 1 towards rhodopsin and beta2-AR Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.15 ATP + beta-adrenergic receptor beta2-adrenergic receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor beta-ARK 2 has a 25% lower specific activity than beta-ARK 1 towards rhodopsin and beta2-AR Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + rhodopsin beta-ARK 2 has a 25% lower specific activity than beta-ARK 1 towards rhodopsin and beta2-AR Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.15 ATP + rhodopsin in form of bovine rod outer segments Bos taurus ADP + phosphorhodopsin
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.15 30
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.15 ATP
-
 Bos taurus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.11.15 Bos taurus beta-ARK 2 has a higher isoelectric point than beta-ARK 1
-
 additional information