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Literature summary extracted from

  • Nidetzky, B.; Eis, C.
    alpha-Retaining glucosyl transfer catalysed by trehalose phosphorylase from Schizophyllum commune: mechanistic evidence obtained from steady-state kinetic studies with substrate analogues and inhibitors (2001), Biochem. J., 360, 727-736.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.231 1-deoxynojirimycin
-
 Schizophyllum commune
2.4.1.231 2-amino-2-deoxy-D-glucose competitive with glucose Schizophyllum commune
2.4.1.231 D-glucal
-
 Schizophyllum commune
2.4.1.231 D-glucono-delta-lactone
-
 Schizophyllum commune
2.4.1.231 isofagomine
-
 Schizophyllum commune
2.4.1.231 orthovanadate competitive against phosphate and alpha-D-glucopyranosyl phosphate, inhibits forward and reverse reaction Schizophyllum commune

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.231 31
-
 alpha-D-glucose 30°C, pH 6.6 Schizophyllum commune
2.4.1.231 87
-
 alpha,alpha-trehalose 30°C, pH 6.6 Schizophyllum commune

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.231 alpha,alpha-trehalose + phosphate Schizophyllum commune
-
 alpha-D-glucose + alpha-D-glucose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.231 Schizophyllum commune
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.231 alpha,alpha-trehalose + phosphate
-
 Schizophyllum commune alpha-D-glucose + alpha-D-glucose 1-phosphate
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.231 10.3
-
 alpha-D-glucose 30°C, pH 6.6 Schizophyllum commune
2.4.1.231 14.1
-
 alpha,alpha-trehalose 30°C, pH 6.6 Schizophyllum commune

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.231 1
-
 orthovanadate 30°C, pH 6.6 Schizophyllum commune
2.4.1.231 46
-
 2-amino-2-deoxy-D-glucose 30°C, pH 6.6 Schizophyllum commune