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Literature summary extracted from

  • Han, S.E.; Kwon, H.B.; Lee, S.B.; Yi, B.Y.; Murayama, I.; Kitamoto, Y.; Byun, M.O.
    Cloning and characterization of a gene encoding trehalose phosphorylase (TP) from Pleurotus sajor-caju (2003), Protein Expr. Purif., 30, 194-202.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.231
-
 Lentinus sajor-caju

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.231 5.4
-
 phosphate pH 7 Lentinus sajor-caju
2.4.1.231 34.3
-
 alpha-D-glucose pH 6.3 Lentinus sajor-caju
2.4.1.231 44.6
-
 alpha-D-glucose 1-phosphate pH 6.3 Lentinus sajor-caju
2.4.1.231 74.8
-
 alpha,alpha-trehalose pH 7 Lentinus sajor-caju

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.231 88000
-
 SDS-PAGE Lentinus sajor-caju

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.231 alpha,alpha-trehalose + phosphate Lentinus sajor-caju
-
 alpha-D-glucose + alpha-D-glucose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.231 Lentinus sajor-caju Q9UV63
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.231
-
 Lentinus sajor-caju

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.231 mycelium
-
 Lentinus sajor-caju
-
2.4.1.231 pileus
-
 Lentinus sajor-caju
-
2.4.1.231 stipe
-
 Lentinus sajor-caju
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.231 13.9
-
-
 Lentinus sajor-caju

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.231 alpha,alpha-trehalose + phosphate
-
 Lentinus sajor-caju alpha-D-glucose + alpha-D-glucose 1-phosphate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.231 36
-
-
 Lentinus sajor-caju

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.231 30
-
 stable below Lentinus sajor-caju
2.4.1.231 40
-
 decrease of activity Lentinus sajor-caju

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.231 6.7
-
 phosphorolytic reaction Lentinus sajor-caju

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.231 6 7 stable Lentinus sajor-caju