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Literature summary extracted from

  • Watts, C.A.; Ridley, H.; Condie, K.L.; Leaver, J.T.; Richardson, D.J.; Butler, C.S.
    Selenate reduction by Enterobacter cloacae SLD1a-1 is catalysed by a molybdenum-dependent membrane-bound enzyme that is distinct from the membrane-bound nitrate reductase (2003), FEMS Microbiol. Lett., 228, 273-279.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.97.1.9 additional information no inhibition by sodium azide Enterobacter cloacae
1.97.1.9 tungstate in vitro and in vivo Enterobacter cloacae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.97.1.9 6.25
-
 benzyl viologen 20°C Enterobacter cloacae
1.97.1.9 6.25
-
 selenate with reduced benzyl viologen, 20°C Enterobacter cloacae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.97.1.9 cytoplasmic membrane with localisation of the catalytic site to the periplasmic side of the membrane Enterobacter cloacae
-
-
1.97.1.9 membrane
-
 Enterobacter cloacae 16020
-
1.97.1.9 additional information not in the cytosol Enterobacter cloacae
-
-
1.97.1.9 periplasm
-
 Enterobacter cloacae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.97.1.9 Molybdenum required Enterobacter cloacae
1.97.1.9 Molybdenum enhances the activity 10fold in vivo when added to the growth medium Enterobacter cloacae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.97.1.9 selenate + reduced acceptor Enterobacter cloacae detoxification of selenate selenite + H2O + acceptor
-
 ?
1.97.1.9 selenate + reduced acceptor Enterobacter cloacae SLD1a-1 / ATCC 700258 detoxification of selenate selenite + H2O + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.97.1.9 Enterobacter cloacae
-
-
-
1.97.1.9 Enterobacter cloacae
-
 highest activity when molybdate is added to the culture medium
-
1.97.1.9 Enterobacter cloacae SLD1a-1 / ATCC 700258
-
-
-
1.97.1.9 Enterobacter cloacae SLD1a-1 / ATCC 700258
-
 highest activity when molybdate is added to the culture medium
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.97.1.9 0.29
-
 membrane fraction Enterobacter cloacae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.97.1.9 selenate + electron donor
-
 Enterobacter cloacae selenite + H2O + oxidized electron donor
-
 ?
1.97.1.9 selenate + electron donor
-
 Enterobacter cloacae SLD1a-1 / ATCC 700258 selenite + H2O + oxidized electron donor
-
 ?
1.97.1.9 selenate + reduced acceptor detoxification of selenate Enterobacter cloacae selenite + H2O + acceptor
-
 ?
1.97.1.9 selenate + reduced acceptor detoxification of selenate Enterobacter cloacae SLD1a-1 / ATCC 700258 selenite + H2O + acceptor
-
 ?
1.97.1.9 selenate + reduced benzyl viologen
-
 Enterobacter cloacae selenite + H2O + oxidized benzyl viologen
-
 ?
1.97.1.9 selenate + reduced benzyl viologen
-
 Enterobacter cloacae SLD1a-1 / ATCC 700258 selenite + H2O + oxidized benzyl viologen
-
 ?
1.97.1.9 selenate + reduced benzyl viologen
-
 Enterobacter cloacae selenite + H2O + benzyl viologen
-
 ?
1.97.1.9 selenate + reduced benzyl viologen
-
 Enterobacter cloacae SLD1a-1 / ATCC 700258 selenite + H2O + benzyl viologen
-
 ?
1.97.1.9 selenate + reduced methyl viologen preferred electron donor Enterobacter cloacae selenite + H2O + oxidized methyl viologen
-
 ?
1.97.1.9 selenate + reduced methyl viologen preferred electron donor Enterobacter cloacae SLD1a-1 / ATCC 700258 selenite + H2O + oxidized methyl viologen
-
 ?
1.97.1.9 selenate + reduced methyl viologen
-
 Enterobacter cloacae selenite + H2O + methyl viologen
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.97.1.9 20
-
 assay at Enterobacter cloacae