BRENDA - Enzyme Database

Characterization of xanthine dehydrogenase from the anaerobic bacterium Veillonella atypica and identification of molybdopterin-cytosine-dinucleotide-containing molybdenum cofactor

Gremer, L.; Meyer, O.; Eur. J. Biochem. 238, 862-866 (1996) View publication on PubMed

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.1.4
0.017
-
xanthine
electron acceptor: ferricyanide
Veillonella atypica
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.17.1.4
Iron-sulfur-center
1 Fe2-S2-cluster
Veillonella atypica
1.17.1.4
molybdenum-center
molybdopterin cytosine dinucleotide cofactor
Veillonella atypica
1.17.1.4
Zn
1.54 mol Zn per mol enzyme
Veillonella atypica
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.17.1.4
18400
-
1 * 82400 + 1 * 28500 + 1 * 18400, SDS-PAGE
Veillonella atypica
1.17.1.4
28500
-
1 * 82400 + 1 * 28500 + 1 * 18400, SDS-PAGE
Veillonella atypica
1.17.1.4
82400
-
1 * 82400 + 1 * 28500 + 1 * 18400, SDS-PAGE
Veillonella atypica
1.17.1.4
129000
-
native PAGE
Veillonella atypica
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.17.1.4
xanthine + O2 + H2O
Veillonella atypica
very low activity
urate + O2- + 2 H+
-
Veillonella atypica
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.17.1.4
Veillonella atypica
-
formerly Micrococcus lactilyticus or Veillonella alcalescens
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.17.1.4
to homogeneity, chromatography techniques
Veillonella atypica
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.17.1.4
cell culture
inducible by xanthine
Veillonella atypica
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.17.1.4
72.56
-
xanthine-ferricyanide assay
Veillonella atypica
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.17.1.4
acetaldehyde + ferricyanide + H2O
5% of the activity compared to xanthine
644570
Veillonella atypica
acetic acid + ferrocyanide
-
644570
Veillonella atypica
?
1.17.1.4
adenine + ferricyanide + H2O
12% of the activity compared to xanthine
644570
Veillonella atypica
urate + ferrocyanide
-
644570
Veillonella atypica
?
1.17.1.4
benzaldehyde + 2 ferricyanide + H2O
4% of the activity compared to xanthine
644570
Veillonella atypica
benzoate + 2 ferrocyanide + 2 H+
-
644570
Veillonella atypica
?
1.17.1.4
hypoxanthine + ferricyanide + H2O
98% of the activity compared to xanthine
644570
Veillonella atypica
xanthine + ferrocyanide
-
644570
Veillonella atypica
?
1.17.1.4
purine + ferricyanide + H2O
108% of the activity compared to xanthine
644570
Veillonella atypica
urate + ferrocyanide
-
644570
Veillonella atypica
?
1.17.1.4
salicylaldehyde + ferricyanide + H2O
2% of the activity compared to xanthine
644570
Veillonella atypica
salicylate + ferrocyanide
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + 2,6-dichlorophenolindophenol + H2O
13% of the activity compared to methylene blue as electron acceptor
644570
Veillonella atypica
urate + ?
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + benzyl viologen + H2O
18% of the activity compared to methylene blue as electron acceptor
644570
Veillonella atypica
urate + reduced benzyl viologen
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + cytochrome c + H2O
low activity
644570
Veillonella atypica
urate + reduced cytochrome c
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + FAD + H2O
35% of the activity compared to methylene blue as electron acceptor
644570
Veillonella atypica
urate + FADH2
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + ferricyanide + H2O
-
644570
Veillonella atypica
urate + ferrocyanide
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + FMN + H2O
44% of the activity compared to methylene blue as electron acceptor
644570
Veillonella atypica
urate + FMNH2
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + iodonitrotetrazolium + H2O
-
644570
Veillonella atypica
urate + reduced iodonitrotetrazolium
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + methyl viologen + H2O
low activity
644570
Veillonella atypica
urate + reduced methyl viologen
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + methylene blue + H2O
-
644570
Veillonella atypica
urate + reduced methylene blue
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + nitroblue tetrazolium + H2O
-
644570
Veillonella atypica
urate + ?
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + O2 + H2O
very low activity
644570
Veillonella atypica
urate + O2- + 2 H+
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + phenazine methosulfate + H2O
-
644570
Veillonella atypica
urate + ?
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + riboflavin + H2O
41% of the activity compared to methylene blue as electron acceptor
644570
Veillonella atypica
urate + reduced riboflavin
-
644570
Veillonella atypica
?
Subunits
EC Number
Subunits
Commentary
Organism
1.17.1.4
trimer
1 * 82400 + 1 * 28500 + 1 * 18400, SDS-PAGE
Veillonella atypica
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.17.1.4
FAD
-
Veillonella atypica
1.17.1.4
additional information
molybdoironflavoprotein: molar ratio of molybdenum to iron to acid-labile-sulfur to FAD is 1 : 2 : 1.9 : 0.8
Veillonella atypica
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.17.1.4
FAD
-
Veillonella atypica
1.17.1.4
additional information
molybdoironflavoprotein: molar ratio of molybdenum to iron to acid-labile-sulfur to FAD is 1 : 2 : 1.9 : 0.8
Veillonella atypica
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.1.4
0.017
-
xanthine
electron acceptor: ferricyanide
Veillonella atypica
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.17.1.4
Iron-sulfur-center
1 Fe2-S2-cluster
Veillonella atypica
1.17.1.4
molybdenum-center
molybdopterin cytosine dinucleotide cofactor
Veillonella atypica
1.17.1.4
Zn
1.54 mol Zn per mol enzyme
Veillonella atypica
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.17.1.4
18400
-
1 * 82400 + 1 * 28500 + 1 * 18400, SDS-PAGE
Veillonella atypica
1.17.1.4
28500
-
1 * 82400 + 1 * 28500 + 1 * 18400, SDS-PAGE
Veillonella atypica
1.17.1.4
82400
-
1 * 82400 + 1 * 28500 + 1 * 18400, SDS-PAGE
Veillonella atypica
1.17.1.4
129000
-
native PAGE
Veillonella atypica
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.17.1.4
xanthine + O2 + H2O
Veillonella atypica
very low activity
urate + O2- + 2 H+
-
Veillonella atypica
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.17.1.4
to homogeneity, chromatography techniques
Veillonella atypica
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.17.1.4
cell culture
inducible by xanthine
Veillonella atypica
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.17.1.4
72.56
-
xanthine-ferricyanide assay
Veillonella atypica
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.17.1.4
acetaldehyde + ferricyanide + H2O
5% of the activity compared to xanthine
644570
Veillonella atypica
acetic acid + ferrocyanide
-
644570
Veillonella atypica
?
1.17.1.4
adenine + ferricyanide + H2O
12% of the activity compared to xanthine
644570
Veillonella atypica
urate + ferrocyanide
-
644570
Veillonella atypica
?
1.17.1.4
benzaldehyde + 2 ferricyanide + H2O
4% of the activity compared to xanthine
644570
Veillonella atypica
benzoate + 2 ferrocyanide + 2 H+
-
644570
Veillonella atypica
?
1.17.1.4
hypoxanthine + ferricyanide + H2O
98% of the activity compared to xanthine
644570
Veillonella atypica
xanthine + ferrocyanide
-
644570
Veillonella atypica
?
1.17.1.4
purine + ferricyanide + H2O
108% of the activity compared to xanthine
644570
Veillonella atypica
urate + ferrocyanide
-
644570
Veillonella atypica
?
1.17.1.4
salicylaldehyde + ferricyanide + H2O
2% of the activity compared to xanthine
644570
Veillonella atypica
salicylate + ferrocyanide
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + 2,6-dichlorophenolindophenol + H2O
13% of the activity compared to methylene blue as electron acceptor
644570
Veillonella atypica
urate + ?
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + benzyl viologen + H2O
18% of the activity compared to methylene blue as electron acceptor
644570
Veillonella atypica
urate + reduced benzyl viologen
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + cytochrome c + H2O
low activity
644570
Veillonella atypica
urate + reduced cytochrome c
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + FAD + H2O
35% of the activity compared to methylene blue as electron acceptor
644570
Veillonella atypica
urate + FADH2
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + ferricyanide + H2O
-
644570
Veillonella atypica
urate + ferrocyanide
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + FMN + H2O
44% of the activity compared to methylene blue as electron acceptor
644570
Veillonella atypica
urate + FMNH2
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + iodonitrotetrazolium + H2O
-
644570
Veillonella atypica
urate + reduced iodonitrotetrazolium
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + methyl viologen + H2O
low activity
644570
Veillonella atypica
urate + reduced methyl viologen
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + methylene blue + H2O
-
644570
Veillonella atypica
urate + reduced methylene blue
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + nitroblue tetrazolium + H2O
-
644570
Veillonella atypica
urate + ?
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + O2 + H2O
very low activity
644570
Veillonella atypica
urate + O2- + 2 H+
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + phenazine methosulfate + H2O
-
644570
Veillonella atypica
urate + ?
-
644570
Veillonella atypica
?
1.17.1.4
xanthine + riboflavin + H2O
41% of the activity compared to methylene blue as electron acceptor
644570
Veillonella atypica
urate + reduced riboflavin
-
644570
Veillonella atypica
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.17.1.4
trimer
1 * 82400 + 1 * 28500 + 1 * 18400, SDS-PAGE
Veillonella atypica