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Literature summary extracted from

  • Rowley, B.; Tucci, A.F.
    Homoisocitric dehydrogenase from yeast (1970), Arch. Biochem. Biophys., 141, 499-510.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.87 2-oxoadipate required for reductive carboxylation Saccharomyces cerevisiae
1.1.1.87 NaHCO3-CO2 required for reductive carboxylation Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.87 homoisocitrate 1.2 mM Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.87 0.01
-
 homoisocitrate value below Saccharomyces cerevisiae
1.1.1.87 0.065
-
 NADH
-
 Saccharomyces cerevisiae
1.1.1.87 0.33
-
 NAD+
-
 Saccharomyces cerevisiae
1.1.1.87 1.5
-
 alpha-ketoadipate
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.87 K+ required for optimal activity Saccharomyces cerevisiae
1.1.1.87 Mg2+ stimulation of oxidative decarboxylation, required for reductive carboxylation Saccharomyces cerevisiae
1.1.1.87 Mn2+ stimulation of oxidative decarboxylation Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.87 48000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.87 1-hydroxy-1,2,4-butanetricarboxylate + NAD+ Saccharomyces cerevisiae
-
 2-oxoadipate + CO2 + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.87 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.87
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.87 3.8
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.87 1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
 Saccharomyces cerevisiae 2-oxoadipate + CO2 + NADH
-
 r
1.1.1.87 1-hydroxy-1,2,4-butanetricarboxylate + NAD+ i.e. homoisocitrate Saccharomyces cerevisiae 2-oxoadipate + CO2 + NADH
-
 r
1.1.1.87 additional information catalyzes keto-enol tautomerization of tritiated alpha-ketoadipate, not: ethanol, isocitrate, malate, glutamate Saccharomyces cerevisiae ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.87 30
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.87 7
-
 reductive carboxylation Saccharomyces cerevisiae
1.1.1.87 8.3 8.8 oxidative decarboxylation Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.87 6.1 7.7 pH 6.1: about 60% of activity maximum, pH 7.7: about 50% of activity maximum, reductive carboxylation Saccharomyces cerevisiae
1.1.1.87 7.4 9.3 pH 7.4: about 50% of activity maximum, pH 9.3: about 90% of activity maximum, oxidative decarboxylation Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.87 NAD+
-
 Saccharomyces cerevisiae
1.1.1.87 NADH
-
 Saccharomyces cerevisiae