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Literature summary extracted from

  • Michel, G.; Roszak, A.W.; Sauvé, V.; Maclean, J.; Matte, A.; Coggins, J.R.; Cygler, M.; Lapthorn, A.J.
    Structures of shikimate dehydrogenase AroE and its paralog YdiB. A common structural framework for different activitie (2003), J. Biol. Chem., 278, 19463-19472.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.282 YdiB, with bound cofactors NAD+ or NADP+ Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.282 0.02
-
 shikimate pH 9, 20°C, cosubstrate NAD+ Escherichia coli
1.1.1.282 0.041
-
 L-quinate pH 9, 20°C, cosubstrate NAD+ Escherichia coli
1.1.1.282 0.087
-
 NAD+ pH 9, 20°C, cosubstrate shikimate Escherichia coli
1.1.1.282 0.1
-
 NADP+ pH 9, 20°C, cosubstrate shikimate Escherichia coli
1.1.1.282 0.116
-
 NAD+ pH 9, 20°C, cosubstrate L-quinate Escherichia coli
1.1.1.282 0.12
-
 shikimate pH 9, 20°C, cosubstrate NADP+ Escherichia coli
1.1.1.282 0.5
-
 NADP+ pH 9, 20°C, cosubstrate L-quinate Escherichia coli
1.1.1.282 0.555
-
 L-quinate pH 9, 20°C, cosubstrate NADP+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.282 64000
-
 apoprotein, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.282 additional information Escherichia coli YdiB may be involved in shikimate pathway or may be essential for growth of the organism with quinate as a sole carbon source ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.282 Escherichia coli
-
-
-
1.1.1.282 Escherichia coli P0A6D5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.282
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.282 L-quinate + NAD(P)+ = 3-dehydroquinate + NAD(P)H + H+ reaction mechanism Escherichia coli
a
1.1.1.282 shikimate + NAD(P)+ = 3-dehydroshikimate + NAD(P)H + H+ reaction mechanism Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.282 additional information
-
 YdiB has a low specific activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.282 L-quinate + NAD(P)+ YdiB is a dual specific quinate/shikimate dehydrogenase that utilizes either NAD+ or NADP+ as cofactor, YdiB is equally active with shikimate or quinate, but has a tendency to be more efficient with NAD+ than with NADP+, detailed structure of YdiB, mechanism Escherichia coli 3-dehydroquinate + NAD(P)H + H+
-
 ?
1.1.1.282 L-quinate + NAD+
-
 Escherichia coli 3-dehydroquinate + NADH + H+
-
 r
1.1.1.282 additional information YdiB may be involved in shikimate pathway or may be essential for growth of the organism with quinate as a sole carbon source Escherichia coli ?
-
 ?
1.1.1.282 shikimate + NAD(P)+ YdiB is a dual specific quinate/shikimate dehydrogenase that utilizes either NAD+ or NADP+ as cofactor, YdiB is equally active with shikimate or quinate, but has a tendency to be more efficient with NAD+ than with NADP+, detailed structure of YdiB, mechanism Escherichia coli 3-dehydroshikimate + NAD(P)H + H+ model for 3-dehydroshikimate recognition ?

Subunits

EC Number Subunits Comment Organism
1.1.1.282 dimer
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.282 20
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.282 0.05
-
 NADP+ pH 9, 20°C, cosubstrate L-quinate Escherichia coli
1.1.1.282 0.05
-
 shikimate pH 9, 20°C, cosubstrate NAD+ Escherichia coli
1.1.1.282 0.05
-
 L-quinate pH 9, 20°C, cosubstrate NAD+ or NADP+ Escherichia coli
1.1.1.282 0.05
-
 NAD+ pH 9, 20°C, cosubstrate shikimate or L-quinate Escherichia coli
1.1.1.282 0.117
-
 shikimate pH 9, 20°C, cosubstrate NADP+ Escherichia coli
1.1.1.282 0.117
-
 NADP+ pH 9, 20°C, cosubstrate shikimate Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.282 9
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.282 NAD+ utilizes either NAD+ or NADP+ as a cofactor, tendency to be more efficient with NAD+ than with NADP+, mode of binding Escherichia coli
1.1.1.282 NADP+ utilizes either NAD+ or NADP+ as a cofactor, tendency to be more efficient with NAD+ than with NADP+, mode of binding Escherichia coli