Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kanoh, H.; Ohno, K.
    1,2-Diacylglycerol: CDPcholine cholinephosphotransferase (1981), Methods Enzymol., 71, 536-546.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.8.2 deoxycholate stimulates, inhibition above 2 mM Rattus norvegicus
2.7.8.2 phosphatidylcholine activates Rattus norvegicus
2.7.8.2 Phospholipids total microsomal phospholipids stimulate Rattus norvegicus
2.7.8.2 taurocholate stimulates Rattus norvegicus

General Stability

EC Number General Stability Organism
2.7.8.1 stable if treated with 0.5% Triton X-100 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.2 CDPethanolamine
-
 Rattus norvegicus
2.7.8.2 CMPcholine product inhibition Rattus norvegicus
2.7.8.2 deoxycholate stimulates, inhibition above 2 mM Rattus norvegicus
2.7.8.2 Triton X-100 activity assayed with Mg2+ is more labile than that assayed with Mn2+; X-100 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.1 0.022
-
 CDP-ethanolamine 30°C, pH 8.0 Rattus norvegicus
2.7.8.1 0.063
-
 diacylglycerol 30°C, pH 8.0 Rattus norvegicus
2.7.8.1 0.14
-
 CMP 30°C, pH 8.0 Rattus norvegicus
2.7.8.2 additional information
-
 additional information
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.1 microsome cytoplasmic surface Rattus norvegicus
-
-
2.7.8.2 microsome cytoplasmic face of microsomal vesicles Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.1 Mg2+ can partially replace Mn2+ in activation Rattus norvegicus
2.7.8.1 Mn2+ required Rattus norvegicus
2.7.8.1 Mn2+ much more effective than Mg2+ Rattus norvegicus
2.7.8.2 Mg2+ required Rattus norvegicus
2.7.8.2 Mn2+ can partially replace Mg2+ in activation Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.1 Rattus norvegicus
-
-
-
2.7.8.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.1
-
 Rattus norvegicus
2.7.8.2
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.8.1 liver
-
 Rattus norvegicus
-
2.7.8.2 brain
-
 Rattus norvegicus
-
2.7.8.2 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.1 additional information
-
-
 Rattus norvegicus
2.7.8.2 additional information
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.8.1 below -20°C, stable for more than 1 month, microsomal preparation Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.1 CDP-ethanolamine + 1,2-diacylglycerol
-
 Rattus norvegicus CMP + a phosphatidylethanolamine
-
 r
2.7.8.1 additional information several 1-oleoyl-2-saturated types of diacylglycerol are effectively used Rattus norvegicus ?
-
 ?
2.7.8.1 additional information fully saturated diacylglycerols like dipalmitoylglycerol are poorly utilized Rattus norvegicus ?
-
 ?
2.7.8.1 additional information 1-acyl-2-oleoylglycerols ranging from 1-lauroyl to 1-heptadecanoyl species: 1-heptadecanoyl and 1-stearoyl species used most actively Rattus norvegicus ?
-
 ?
2.7.8.1 phosphatidylcholine + CMP
-
 Rattus norvegicus CDP-choline + diacylglycerol
-
 ?
2.7.8.2 CDP-choline + 1,2-diacylglycerol
-
 Rattus norvegicus CMP + a phosphatidylcholine
-
 r
2.7.8.2 CDP-choline + 1,2-dipalmitoyl-sn-glycerol poor substrate Rattus norvegicus CMP + 1,2-dipalmitoyl-sn-glycero-3-phosphocholine
-
 ?
2.7.8.2 CMP + phosphatidylcholine
-
 Rattus norvegicus CDP-choline + 1,2-diacylglycerol
-
 r
2.7.8.2 additional information 1-acyl-2-oleoylglycerols, ranging from 1-lauroyl to 1-heptadecanoyl species are equally well utilized, fully saturated diacylglycerols like dipalmitoylglycerol are poorly utilized, several 1-oleoyl-2-saturated types of diacylglycerol are effectively used Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.8.1 additional information
-
 temperature dependence Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.1 8 8.5
-
 Rattus norvegicus
2.7.8.2 8 8.5
-
 Rattus norvegicus