EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.8.2.25 | additional information | EDTA, SH-reagents at 1 and 10 mM, e.g. p-chloromercuribenzoate, iodoacetate or iodoacetamide, are not inhibitory | Flaveria chlorifolia | |
2.8.2.26 | additional information | EDTA up to 10 mM, or SH-group reagents, e.g. p-chloromercuribenzoate, iodoacetate, iodoacetamide at 1 and 10 mM, are not inhibitory | Flaveria chlorifolia | |
2.8.2.27 | additional information | EDTA up to 10 mM, SH-group reagents, e.g. p-chloromercuribenzoate, iodoacetate, iodoacetamide, all at 1 and 10 mM, are not inhibitory | Flaveria chlorifolia | |
2.8.2.27 | phosphate buffer | complete inhibition | Flaveria chlorifolia |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.2.25 | 0.0002 | - |
quercetin | 30°C, pH 7.5 | Flaveria chlorifolia | |
2.8.2.25 | 0.0002 | - |
3'-phosphoadenylylsulfate | cosubstrate quercetin, pH 7.5, 30°C | Flaveria chlorifolia | |
2.8.2.26 | 0.00029 | - |
quercetin 3-sulfate | - |
Flaveria chlorifolia | |
2.8.2.26 | 0.00035 | - |
3'-phosphoadenylylsulfate | - |
Flaveria chlorifolia | |
2.8.2.27 | 0.00036 | - |
quercetin 3-sulfate | 30°C, pH 7.5 | Flaveria chlorifolia | |
2.8.2.27 | 0.00038 | - |
3'-phosphoadenylylsulfate | 30°C, pH 7.5 | Flaveria chlorifolia |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.8.2.25 | additional information | no divalent cation required | Flaveria chlorifolia | |
2.8.2.27 | additional information | no requirement for divalent cations | Flaveria chlorifolia |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.8.2.25 | 35000 | - |
gel filtration | Flaveria chlorifolia |
2.8.2.26 | 35000 | - |
gel filtration | Flaveria chlorifolia |
2.8.2.27 | 35000 | - |
gel filtration | Flaveria chlorifolia |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.2.25 | 3'-phosphoadenylylsulfate + quercetin | Flaveria chlorifolia | - |
adenosine 3',5'-bisphosphate + quercetin 3-sulfate | - |
? | |
2.8.2.25 | additional information | Flaveria chlorifolia | involved in biosynthesis of polysulfated flavonols | ? | - |
? | |
2.8.2.26 | 3'-phosphoadenylylsulfate + quercetin 3-sulfate | Flaveria chlorifolia | - |
adenosine 3',5'-bisphosphate + quercetin 3,3'-bissulfate | - |
? | |
2.8.2.27 | 3'-phosphoadenylylsulfate + quercetin 3-sulfate | Flaveria chlorifolia | - |
quercetin 3,4'-disulfate + adenosine 3',5'-bisphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.2.25 | Flaveria chlorifolia | - |
- |
- |
2.8.2.26 | Flaveria chlorifolia | - |
- |
- |
2.8.2.27 | Flaveria chlorifolia | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.8.2.25 | partial | Flaveria chlorifolia |
2.8.2.26 | partial | Flaveria chlorifolia |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.8.2.25 | shoot tip | - |
Flaveria chlorifolia | - |
2.8.2.26 | shoot tip | - |
Flaveria chlorifolia | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.8.2.25 | 0.00027 | - |
- |
Flaveria chlorifolia |
2.8.2.26 | 0.0025 | - |
- |
Flaveria chlorifolia |
2.8.2.27 | 0.002 | - |
30°C, pH 7.5 | Flaveria chlorifolia |
EC Number | Storage Stability | Organism |
---|---|---|
2.8.2.26 | -20°C, 1 mg/ml bovine serum albumin, half-life of 3 days | Flaveria chlorifolia |
2.8.2.26 | 4°C, half-life of 24 h | Flaveria chlorifolia |
2.8.2.27 | -20°C, bovine serum albumin up to 1 mg/ml, half-life of 3 days | Flaveria chlorifolia |
2.8.2.27 | 4°C, half-life of 24 h | Flaveria chlorifolia |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.2.25 | 3'-phosphoadenylylsulfate + isorhamnetin | 94% of activity compared to rhamnetin | Flaveria chlorifolia | adenosine 3',5'-bisphosphate + isorhamnetin 3-sulfate | - |
? | |
2.8.2.25 | 3'-phosphoadenylylsulfate + kaempferol | 48% of activity compared to rhamnetin | Flaveria chlorifolia | adenosine 3',5'-bisphosphate + kaempferol 3-sulfate | - |
? | |
2.8.2.25 | 3'-phosphoadenylylsulfate + ombuin | 37% of activity compared to rhamnetin | Flaveria chlorifolia | adenosine 3',5'-bisphosphate + ? | - |
? | |
2.8.2.25 | 3'-phosphoadenylylsulfate + patuletin | 52% of activity compared to rhamnetin | Flaveria chlorifolia | adenosine 3',5'-bisphosphate + patuletin 3-sulfate | - |
? | |
2.8.2.25 | 3'-phosphoadenylylsulfate + quercetin | - |
Flaveria chlorifolia | adenosine 3',5'-bisphosphate + quercetin 3-sulfate | - |
? | |
2.8.2.25 | 3'-phosphoadenylylsulfate + quercetin | 58% of activity compared to rhamnetin | Flaveria chlorifolia | adenosine 3',5'-bisphosphate + quercetin 3-sulfate | - |
? | |
2.8.2.25 | 3'-phosphoadenylylsulfate + rhamnetin | best substrate | Flaveria chlorifolia | adenosine 3',5'-bisphosphate + rhamnetin 3-sulfate | - |
? | |
2.8.2.25 | 3'-phosphoadenylylsulfate + tamarixetin | 31% of activity compared to rhamnetin | Flaveria chlorifolia | adenosine 3',5'-bisphosphate + ? | - |
? | |
2.8.2.25 | additional information | quercetagetin, gossypetin, myricetin or galangin are no substrates | Flaveria chlorifolia | ? | - |
? | |
2.8.2.25 | additional information | involved in biosynthesis of polysulfated flavonols | Flaveria chlorifolia | ? | - |
? | |
2.8.2.26 | 3'-phosphoadenylylsulfate + patuletin 3-sulfate | 33% of activity compared to quercetin 3-sulfate | Flaveria chlorifolia | adenosine 3',5'-bisphosphate + ? | - |
? | |
2.8.2.26 | 3'-phosphoadenylylsulfate + quercetin 3-sulfate | - |
Flaveria chlorifolia | adenosine 3',5'-bisphosphate + quercetin 3,3'-bissulfate | - |
? | |
2.8.2.26 | 3'-phosphoadenylylsulfate + tamarixetin 3-sulfate | 33% of activity compared to quercetin 3-sulfate | Flaveria chlorifolia | adenosine 3',5'-bisphosphate + ? | - |
? | |
2.8.2.26 | additional information | kaempferol 3-sulfate, isorhamnetin 3-sulfate or other flavonol aglycones are no substrates | Flaveria chlorifolia | ? | - |
? | |
2.8.2.27 | 3'-phosphoadenylylsulfate + isorhamnetin 3-sulfate | 38% of activity compared to quercetin 3-sulfate | Flaveria chlorifolia | isorhamnetin 3,4'-disulfate + adenosine 3',5'-bisphosphate | - |
? | |
2.8.2.27 | 3'-phosphoadenylylsulfate + kaempferol 3-sulfate | 45% of activity compared to quercetin 3-sulfate | Flaveria chlorifolia | kaempferol 3,4'-disulfate + adenosine 3',5'-bisphosphate | - |
? | |
2.8.2.27 | 3'-phosphoadenylylsulfate + patuletin 3-sulfate | 12% of activity compared to quercetin 3-sulfate | Flaveria chlorifolia | patuletin 3,4'-disulfate + adenosine 3',5'-bisphosphate | - |
? | |
2.8.2.27 | 3'-phosphoadenylylsulfate + quercetin 3-sulfate | - |
Flaveria chlorifolia | quercetin 3,4'-disulfate + adenosine 3',5'-bisphosphate | - |
? | |
2.8.2.27 | additional information | tamarixetin 3-sulfate, flavonol aglycones, or adenosine 5'-phosphosulfate are no substrates | Flaveria chlorifolia | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.8.2.25 | 30 | - |
assay at | Flaveria chlorifolia |
2.8.2.27 | 30 | - |
assay at | Flaveria chlorifolia |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.8.2.25 | 6.5 | - |
and pH 8.5, two maxima | Flaveria chlorifolia |
2.8.2.25 | 8.5 | - |
and pH 6.5, two maxima | Flaveria chlorifolia |
2.8.2.26 | 7.5 | - |
- |
Flaveria chlorifolia |
2.8.2.27 | 7.5 | - |
- |
Flaveria chlorifolia |