Literature summary extracted from
Nakai, T.; Mizutani, H.; Miyahara, I.; Hirotsu, K.; Takeda, S.; Jhee, K.H.; Yoshimura, T.; Esaki, N.
Three-dimensional structure of 4-amino-4-deoxychorismate lyase from Escherichia coli (2000), J. Biochem., 128, 29-38.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.1.3.38 |
expression in Escherichia coli JM109 |
Escherichia coli |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
4.1.3.38 |
small yellow prisms crystals in the unliganded form obtained using the sparse-matrix method along with the hanging-drop vapor-difussion method |
Escherichia coli |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
4.1.3.38 |
29715 |
- |
2 * 29715 |
Escherichia coli |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.1.3.38 |
Escherichia coli |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.1.3.38 |
fractionation with ammonium sulfate, DEAE-Toyopearl column, butyl-Toyopearl column and Mono Q column |
Escherichia coli |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.1.3.38 |
4-amino-4-deoxychorismate |
re-face specificity |
Escherichia coli |
4-aminobenzoate + pyruvate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.1.3.38 |
dimer |
2 * 29715 |
Escherichia coli |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
4.1.3.38 |
pyridoxal 5'-phosphate |
tightly bound |
Escherichia coli |
|