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Literature summary extracted from

  • Harmark, K.; Anborgh, P.H.; Merola, M.; Clark, B.F.C.; Parmeggiani, A.
    Substitution of aspartic acid-80, a residue involved in coordination of Magnesium, weakens the GTP binding and strongly enhances the GTPase of the G domain of elongations factor Tu (1992), Biochemistry, 31, 7367-7372.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.5.3 kirromycin
-
 Escherichia coli
3.6.5.3 NH4+
-
 Escherichia coli
3.6.5.3 ribosomal subunits
-
 Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.5.3
-
 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.5.3
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.6.5.3 D80N mutant with decreased affinity for GTP and increased GTPase activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.5.3 EDTA
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.5.3 0.0008
-
 GTP wild type Escherichia coli
3.6.5.3 0.016
-
 GTP D80N Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.5.3 K+
-
 Escherichia coli
3.6.5.3 Li+
-
 Escherichia coli
3.6.5.3 Mg2+
-
 Escherichia coli
3.6.5.3 Mn2+
-
 Escherichia coli
3.6.5.3 Na+
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.5.3 GDP + H2O Escherichia coli
-
 ?
-
 ?
3.6.5.3 GDP + H2O Escherichia coli N4830
-
 ?
-
 ?
3.6.5.3 GTP + H2O Escherichia coli
-
 GDP + phosphate
-
 ?
3.6.5.3 GTP + H2O Escherichia coli N4830
-
 GDP + phosphate
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.6.5.3 Glycerol
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.6.5.3 Escherichia coli
-
 elongation factor EF-Tu (wt) and EF-Tu (D80N)
-
3.6.5.3 Escherichia coli N4830
-
 elongation factor EF-Tu (wt) and EF-Tu (D80N)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.5.3
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.5.3 GDP + H2O
-
 Escherichia coli ?
-
 ?
3.6.5.3 GDP + H2O
-
 Escherichia coli N4830 ?
-
 ?
3.6.5.3 GTP + H2O
-
 Escherichia coli GDP + phosphate
-
 ?
3.6.5.3 GTP + H2O
-
 Escherichia coli N4830 GDP + phosphate
-
 ?