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Literature summary extracted from

  • Plankert, U.; Purwin, C.; Holzer, H.
    Yeast fructose-2,6-bisphosphate 6-phosphatase is encoded by PHO8, the gene for nonspecific repressible alkaline phosphatase (1991), Eur. J. Biochem., 196, 191-196.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.54 1,10-phenanthroline
-
 Saccharomyces cerevisiae
3.1.3.54 Neocuproine i.e. 2,9-dimethyl-1,10-phenanthroline, 0.1 mM Zn2+ stabilizes the activity Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.54 vacuole
-
 Saccharomyces cerevisiae 5773
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.54 Cu2+ dimer contains 1.3 mol copper per mol of subunit Saccharomyces cerevisiae
3.1.3.54 Zn2+ dimer contains 1.5 mol zinc per mol of subunit Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.54 D-fructose 2,6-bisphosphate + H2O Saccharomyces cerevisiae
-
 D-fructose 2-phosphate + phosphate
-
 ?
3.1.3.54 D-fructose 2,6-bisphosphate + H2O Saccharomyces cerevisiae M1
-
 D-fructose 2-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.54 Saccharomyces cerevisiae
-
-
-
3.1.3.54 Saccharomyces cerevisiae M1
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.54 glycoprotein
-
 Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.54 7000fold, strain M1 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.54 additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.54 4-nitrophenyl phosphate + H2O
-
 Saccharomyces cerevisiae 4-nitrophenol + phosphate
-
 ?
3.1.3.54 4-nitrophenyl phosphate + H2O
-
 Saccharomyces cerevisiae M1 4-nitrophenol + phosphate
-
 ?
3.1.3.54 D-fructose 2,6-bisphosphate + H2O
-
 Saccharomyces cerevisiae D-fructose 2-phosphate + phosphate
-
 ?
3.1.3.54 D-fructose 2,6-bisphosphate + H2O
-
 Saccharomyces cerevisiae M1 D-fructose 2-phosphate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.54 30
-
 assay at Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.54 30
-
 pH 7.7, about 60% loss of activity after 15 min, fructose-2,6-bisphosphate 6-phosphohydrolase activity Saccharomyces cerevisiae
3.1.3.54 40
-
 pH 7.0, 5 min, stable, fructose-2,6-bisphosphate 6-phosphohydrolase activity Saccharomyces cerevisiae
3.1.3.54 50
-
 pH 7.0, 5 min, about 30% loss of activity, fructose-2,6-bisphosphate 6-phosphohydrolase activity Saccharomyces cerevisiae
3.1.3.54 60
-
 pH 7.0, 5 min, about 70% loss of activity, fructose-2,6-bisphosphate 6-phosphohydrolase activity Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.54 6 6.5 D-fructose 2,6-bisphosphate Saccharomyces cerevisiae
3.1.3.54 6
-
 with D-fructose 2,6-bisphosphate as substrate Saccharomyces cerevisiae
3.1.3.54 9
-
 above, 4-nitrophenyl phosphate Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.54 4 7.5 pH 4: about 25% of activity maximum, pH 7.5: about 20% of activity maximum, fructose 2,6-bisphosphate Saccharomyces cerevisiae