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Literature summary extracted from

  • Purwin, C.; Laux, M.; Holzer, H.
    Fructose 2-phosphate, an intermediate of the dephosphorylation of fructose 2,6-bisphosphate with a purified yeast enzyme (1987), Eur. J. Biochem., 164, 27-30.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.54 diphosphate 50% inhibition at 0.3 mM Saccharomyces cerevisiae
3.1.3.54 phosphate 50% inhibition at 0.9 mM Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.54 0.006
-
 D-fructose 2,6-bisphosphate
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.54 Mg2+ required, half-maximal activity at 0.15 mM Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.54 60000
-
 2 * 60000, SDS-PAGE Saccharomyces cerevisiae
3.1.3.54 120000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.54 D-fructose 2,6-bisphosphate + H2O Saccharomyces cerevisiae
-
 D-fructose 2-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.54 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.54
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.54 additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.54 4-nitrophenyl phosphate + H2O
-
 Saccharomyces cerevisiae 4-nitrophenol + phosphate
-
 ?
3.1.3.54 D-fructose 2,6-bisphosphate + H2O
-
 Saccharomyces cerevisiae D-fructose 2-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.54 dimer 2 * 60000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.54 30
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.54 6
-
 with D-fructose 2,6-bisphosphate as substrate Saccharomyces cerevisiae
3.1.3.54 8
-
 above, 4-nitrophenyl phosphate Saccharomyces cerevisiae