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Literature summary extracted from
- Transfer ribonucleic acid sulfurtransferase isolated from rat cerebral hemispheres (1974), Biochemistry, 13, 2805-2812.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.8.1.4 | 2-mercaptoethanol | requires a thiol for optimal sulfur transfer | Rattus norvegicus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.8.1.4 | 2-mercaptoethanol | - |
Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.8.1.4 | cytoplasm | - |
Rattus norvegicus | 5737 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.8.1.4 | Mg2+ | - |
Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.8.1.4 | L-cysteine + 'activated' tRNA | Rattus norvegicus | - |
L-serine + tRNA containing a thionucleotide | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.8.1.4 | Rattus norvegicus | - |
buffalo | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.8.1.4 | partially | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.8.1.4 | brain | cerebral hemisphere | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.8.1.4 | 0.0000658 | - |
- |
Rattus norvegicus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 2.8.1.4 | -75°C, ammonium sulfate fractionated preparation retains its activity for several months | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.8.1.4 | 3-mercaptopyruvate + activated tRNA | in vitro | Rattus norvegicus | ? + S-tRNA | - |
? | |
| 2.8.1.4 | L-cysteine + 'activated' tRNA | - |
Rattus norvegicus | L-serine + tRNA containing a thionucleotide | - |
r | |
| 2.8.1.4 | L-cysteine + E. coli 'activated' tRNA | - |
Rattus norvegicus | L-serine + tRNA containing a thionucleotide | - |
r | |
| 2.8.1.4 | L-cysteine + rabbit liver 'activated' tRNA | - |
Rattus norvegicus | L-serine + tRNA containing a thionucleotide | - |
r | |
| 2.8.1.4 | L-cysteine + Rattus norvegicus liver 'activated' tRNA | - |
Rattus norvegicus | L-serine + tRNA containing a thionucleotide | - |
r | |
| 2.8.1.4 | L-cysteine + Saccharomyces cerevisiae 'activated' tRNA | - |
Rattus norvegicus | L-serine + tRNA containing a thionucleotide | - |
r | |
| 2.8.1.4 | additional information | rRNA, mRNAand synthetic ribohomopolymers are no acceptor substrates, little or no reaction with E. coli B rRNA, phage MS2 RNA, polyU, polyC, polyA, and polyG | Rattus norvegicus | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.8.1.4 | 7.5 | - |
- |
Rattus norvegicus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.8.1.4 | 7 | 8.5 | - |
Rattus norvegicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.8.1.4 | ATP | - |
Rattus norvegicus | |
| 2.8.1.4 | additional information | sulfur transfer obtained with ADP is apparently due to the formation of ATP by the reaction 2 ADP = ATP + AMP, AMP is totally inactive as activating nucleotide | Rattus norvegicus |
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