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Literature summary extracted from

  • Bretthauer, R.K.; Kozak, L.P.; Irwin, W.E.
    Phosphate and mannose transfer from guanosine diphosphate mannose to yeast mannan acceptors (1969), Biochem. Biophys. Res. Commun., 37, 820-827.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.9 GDP 0.5 mM: 70% inhibition Nakazawaea holstii
2.7.8.9 GMP 0.5 mM: 70% inhibition Nakazawaea holstii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.9 Mn2+ 10 mM: optimum, divalent metal ion required Nakazawaea holstii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.9 GDP-mannose + endogenous acceptor molecule Nakazawaea holstii
-
 GDP + endogenous acceptor molecule modified
-
 ?
2.7.8.9 GDP-mannose + endogenous acceptor molecule Nakazawaea holstii NRRL-Y 2448
-
 GDP + endogenous acceptor molecule modified
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.9 Nakazawaea holstii
-
 NRRL-Y 2448
-
2.7.8.9 Nakazawaea holstii NRRL-Y 2448
-
 NRRL-Y 2448
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.9 partial Nakazawaea holstii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.9 GDP-mannose + endogenous acceptor molecule
-
 Nakazawaea holstii GDP + endogenous acceptor molecule modified
-
 ?
2.7.8.9 GDP-mannose + endogenous acceptor molecule crude extract, acceptor may be cell wall mannan, glycoprotein or exocellular phosphomannan Nakazawaea holstii GDP + endogenous acceptor molecule modified mannose 1-phosphate is transferred from GDP-mannose forming a 1,6'-phosphodiester linkage between 2 mannose residues (proposed) ?
2.7.8.9 GDP-mannose + endogenous acceptor molecule
-
 Nakazawaea holstii NRRL-Y 2448 GDP + endogenous acceptor molecule modified
-
 ?
2.7.8.9 GDP-mannose + endogenous acceptor molecule crude extract, acceptor may be cell wall mannan, glycoprotein or exocellular phosphomannan Nakazawaea holstii NRRL-Y 2448 GDP + endogenous acceptor molecule modified mannose 1-phosphate is transferred from GDP-mannose forming a 1,6'-phosphodiester linkage between 2 mannose residues (proposed) ?
2.7.8.9 additional information mannose 6-phosphate, GDP are ineffective Nakazawaea holstii ?
-
 ?
2.7.8.9 additional information mannose 6-phosphate, GDP are ineffective Nakazawaea holstii NRRL-Y 2448 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.8.9 30
-
 assay at Nakazawaea holstii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.9 6.5
-
 assay at Nakazawaea holstii