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Literature summary extracted from

  • Dowhan, W.
    Phosphatidylserine synthase from Escherichia coli (1992), Methods Enzymol., 209, 287-298.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.8.8 Triton X-100 dependent on nonionic detergent, at 0.1 mM CDP-diacylglycerol optimal activity occurs at a Triton to substrate molar ratio of 8:1 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.8.8 additional information mutants in the pss gene stop growing after 4 to 5 generations at the restruiction temperature 42°C. The enzyme isolated from such mutants is also temperature-labile Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.8 52817
-
 x * 52817, calculation from nucleotide sequence Escherichia coli
2.7.8.8 500000
-
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.8 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.8.8 CDP-diacylglycerol + L-serine = CMP + (3-sn-phosphatidyl)-L-serine two-step ping-pong reaction mechanism involving covalently bound enzyme-phosphatidyl intermediate Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.8 39
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.8 CDP-diacylglycerol + L-Ser
-
 Escherichia coli CMP + 3-O-sn-phosphatidyl-L-serine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.8.8 ? x * 52817, calculation from nucleotide sequence Escherichia coli