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Literature summary extracted from
- Synthesis of the unusual metabolite carboxyphosphoenolpyruvate. Cloning and expression of carboxyphosphonoenolpyruvate mutase (1992), J. Am. Chem. Soc., 114, 377-378.
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 2.7.8.23 | agriculture | formation of one of the two C-P bonds in the tripeptide antibiotic bialaphos, a potent herbicide isolated from Streptomyces hygroscopicus SF1293, enzyme is produced in large amounts by high producer strain HP5-29, enzyme may contribute to improvement of bialphos production | Streptomyces hygroscopicus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.7.8.23 | cloned and expressd at high levels in Escherichia coli | Streptomyces hygroscopicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.7.8.23 | 0.27 | - |
carboxyphosphoenolpyruvate | - |
Streptomyces hygroscopicus |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.7.8.23 | 32000 | - |
2 * 32000, homodimer, SDS-PAGE | Streptomyces hygroscopicus |
| 2.7.8.23 | 32700 | - |
calculated from amino acid composition | Streptomyces hygroscopicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.8.23 | carboxyphosphonoenolpyruvate | Streptomyces hygroscopicus | - |
(hydroxyphosphinyl)pyruvate + CO2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.8.23 | Streptomyces hygroscopicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.8.23 | - |
Streptomyces hygroscopicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.8.23 | carboxyphosphoenolpyruvate | - |
Streptomyces hygroscopicus | ? | - |
? | |
| 2.7.8.23 | carboxyphosphonoenolpyruvate | - |
Streptomyces hygroscopicus | (hydroxyphosphinyl)pyruvate + CO2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.7.8.23 | dimer | 2 * 32000, homodimer, SDS-PAGE | Streptomyces hygroscopicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.7.8.23 | 0.02 | - |
carboxyphosphoenolpyruvate | - |
Streptomyces hygroscopicus | |
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Release 2023.1 (January 2023)
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