EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.B74 | expression in Escherichia coli | Hordeum vulgare |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.B74 | 0.0046 | - |
5,7,4'-trihydroxyflavone | pH 7.8, 20°C | Hordeum vulgare | |
2.1.1.B74 | 0.0109 | - |
S-adenosyl-L-methionine | pH 7.8, 20°C | Hordeum vulgare |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.B74 | 42300 | - |
calculated from sequence | Hordeum vulgare |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.B74 | S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone | Hordeum vulgare | i.e. apigenin. The product genkwanin exhibits a stronger inhibitory effect on the germination of spores from pathogenic fungi as compared to the effect of apigenin | S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone | i.e. genkwanin | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.B74 | Hordeum vulgare | Q43771 | inoculated with the pathogenic fungus Blumeria graminis | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.B74 | - |
Hordeum vulgare |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.B74 | kernel | - |
Hordeum vulgare | - |
2.1.1.B74 | leaf | expressed in barley leaves in response to attack by the pathogenic fungus Blumeria graminis. NOt detected in healthy plants | Hordeum vulgare | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.B74 | S-adenosyl-L-methionine + 3',4',5,7-tetrahydroxyflavanone | i.e. eriodictyol, about 10% of the activity with 5,7,4'-trihydroxyflavone (i.e. apigenin) | Hordeum vulgare | S-adenosyl-L-homocysteine + 3',4',5-trihydroxy-7-methoxyflavanone | - |
? | |
2.1.1.B74 | S-adenosyl-L-methionine + 3,4',5,7-tetrahydroxyflavanone | i.e. kaempferol, about 15% of the activity with 5,7,4'-trihydroxyflavone (i.e. apigenin) | Hordeum vulgare | S-adenosyl-L-homocysteine + 3,4',5-trihydroxy-7-methoxyflavanone | - |
? | |
2.1.1.B74 | S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone | i.e. luteolin, about 15% of the activity with 5,7,4'-trihydroxyflavone (i.e. apigenin) | Hordeum vulgare | S-adenosyl-L-homocysteine + 7-methoxy-5,3',4'-tetrahydroxyflavone | - |
? | |
2.1.1.B74 | S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavanone | i.e. naringenin, about 30% of the activity with 5,7,4'-trihydroxyflavone (i.e. apigenin) | Hordeum vulgare | S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavanone | i.e. sakuranetin | ? | |
2.1.1.B74 | S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone | i.e. apigenin. The product genkwanin exhibits a stronger inhibitory effect on the germination of spores from pathogenic fungi as compared to the effect of apigenin | Hordeum vulgare | S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone | i.e. genkwanin | ? | |
2.1.1.B74 | S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone | i.e. apigenin, sequential bi-bi mechanism | Hordeum vulgare | S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone | i.e. genkwanin | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.B74 | ? | x * 42300, calculated from sequence | Hordeum vulgare |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.B74 | F1-OMT | - |
Hordeum vulgare |
2.1.1.B74 | flavonoid 7-O-methyltransferase | ambiguous | Hordeum vulgare |
2.1.1.B74 | flavonoid 7-OMT | ambiguous | Hordeum vulgare |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.B74 | 20 | - |
assay at | Hordeum vulgare |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.B74 | 7.8 | - |
assay at | Hordeum vulgare |