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Literature summary extracted from

  • Elling, L.
    Kinetic characterization of UDP-glucose pyrophosphorylase from germinated barley (malt) (1996), Phytochemistry, 42, 955-960.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.7.7.9 synthesis easy available enzyme can be used for synthesis of nucleotide sugars in enzymic glycoconjugate synthesis Hordeum vulgare subsp. vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.9 diphosphate noncompetitive to glucose 1-phosphate and UTP Hordeum vulgare subsp. vulgare
2.7.7.9 phosphate noncompetitive to glucose 1-phosphate and UTP Hordeum vulgare subsp. vulgare
2.7.7.9 UDP-glucose competitive to UTP, noncompetitive to glucose 1-phosphate Hordeum vulgare subsp. vulgare
2.7.7.9 UTP competitive to UDP-glucose; product inhibition Hordeum vulgare subsp. vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.9 additional information
-
 additional information kinetics Hordeum vulgare subsp. vulgare
2.7.7.9 0.074
-
 alpha-D-glucose 1-phosphate pH 8.35,25°C Hordeum vulgare subsp. vulgare
2.7.7.9 0.093
-
 UTP pH 8.35, 25°C Hordeum vulgare subsp. vulgare
2.7.7.9 0.17
-
 diphosphate pH 8.35, 25°C Hordeum vulgare subsp. vulgare
2.7.7.9 0.19
-
 UDP-glucose pH 8.35, 25°C Hordeum vulgare subsp. vulgare

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.9 Mg2+ requirement Hordeum vulgare subsp. vulgare

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.9 Hordeum vulgare subsp. vulgare
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.9 UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose sequential ordered bi bi reaction mechanism Hordeum vulgare subsp. vulgare
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.9 seedling germinated, malt Hordeum vulgare subsp. vulgare
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.9 ATP + alpha-D-glucose 1-phosphate 13.7% of the activity with UTP Hordeum vulgare subsp. vulgare diphosphate + ADP-glucose
-
 r
2.7.7.9 diphosphate + CDP-glucose 18.5% of the activity with UTP Hordeum vulgare subsp. vulgare CTP + alpha-D-glucose 1-phosphate
-
 r
2.7.7.9 diphosphate + GDP-glucose 14.3% of the activity with UTP Hordeum vulgare subsp. vulgare GTP + alpha-D-glucose 1-phosphate
-
 r
2.7.7.9 additional information substrate specificity Hordeum vulgare subsp. vulgare ?
-
 ?
2.7.7.9 UTP + alpha-D-glucose 1-phosphate
-
 Hordeum vulgare subsp. vulgare diphosphate + UDP-glucose
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.9 25
-
 assay at Hordeum vulgare subsp. vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.9 8.35
-
 assay at Hordeum vulgare subsp. vulgare

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.9 0.015
-
 UDP-glucose versus glucose 1-phosphate, pH 8.35, 25°C Hordeum vulgare subsp. vulgare
2.7.7.9 0.12
-
 UDP-glucose versus UTP, pH 8.35, 25°C Hordeum vulgare subsp. vulgare
2.7.7.9 0.17
-
 UTP versus UDP-glucose, pH 8.35, 25°C Hordeum vulgare subsp. vulgare
2.7.7.9 0.213
-
 diphosphate versus glucose 1-phosphate, pH 8.35, 25°C Hordeum vulgare subsp. vulgare
2.7.7.9 0.95
-
 diphosphate versus UTP, pH 8.35, 25°C Hordeum vulgare subsp. vulgare
2.7.7.9 10.9
-
 phosphate versus UTP, pH 8.35, 25°C Hordeum vulgare subsp. vulgare
2.7.7.9 12.2
-
 phosphate versus glucose 1-phosphate, pH 8.35, 25°C Hordeum vulgare subsp. vulgare