Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sowokinos, J.R.; Spychalla, J.P.; Desborough, S.L.
    Pyrophosphorylases in Solanum tuberosum. IV. Purification, tissue localization, and physicochemical properties of UDP-glucose pyrophosphorylase (1993), Plant Physiol., 101, 1073-1080.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.9 additional information no activation by 3-phosphoglycerate Solanum tuberosum
2.7.7.9 additional information no activation by fructose 2,6-bisphosphate, UDPglucose synthesis Solanum tuberosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.9 3-phosphoglycerate no inhibition of UDP-glucose synthesis Solanum tuberosum
2.7.7.9 D-fructose 2,6-bisphosphate no inhibition of UDP-glucose synthesis Solanum tuberosum
2.7.7.9 D-fructose 6-phosphate
-
 Solanum tuberosum
2.7.7.9 phosphate
-
 Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.9 additional information
-
 additional information kinetics Solanum tuberosum
2.7.7.9 0.055 0.08 alpha-D-glucose 1-phosphate at physiological substrate concentration, pH 8.5, 37°C Solanum tuberosum
2.7.7.9 0.1 0.2 UTP at physiological substrate concentrations, pH 8.5, 37°C Solanum tuberosum
2.7.7.9 0.11 0.13 diphosphate at physiological substrate concentration, pH 8.5, 37°C Solanum tuberosum
2.7.7.9 0.12 0.18 UDP-glucose at physiological substrate concentrations, pH 8.5, 37°C Solanum tuberosum
2.7.7.9 0.53
-
 UTP at substrate concentrations above 2 mM, pH 8.5, 37°C Solanum tuberosum
2.7.7.9 0.68 1 alpha-D-glucose 1-phosphate at substrate concentrations above 2 mM, pH 8.5, 37°C Solanum tuberosum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.9 Mg2+ requirement Solanum tuberosum
2.7.7.9 Mg2+ 5 mM Solanum tuberosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.9 53000
-
 gel filtration Solanum tuberosum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.9 Solanum tuberosum
-
 cv. Norchip
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.9 177fold to near homogeneity Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.9 cell suspension culture
-
 Solanum tuberosum
-
2.7.7.9 leaf low level and activity Solanum tuberosum
-
2.7.7.9 additional information tissue distribution Solanum tuberosum
-
2.7.7.9 root low level and activity Solanum tuberosum
-
2.7.7.9 stem
-
 Solanum tuberosum
-
2.7.7.9 stolon
-
 Solanum tuberosum
-
2.7.7.9 tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.9 additional information
-
 activity in different tissues Solanum tuberosum
2.7.7.9 1099
-
 purified enzyme Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.9 additional information no activity with ADP-glucose Solanum tuberosum ?
-
 ?
2.7.7.9 additional information no activity with ATP, CTP, and GTP Solanum tuberosum ?
-
 ?
2.7.7.9 UTP + alpha-D-glucose 1-phosphate
-
 Solanum tuberosum diphosphate + UDP-glucose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.9 monomer 1 * 53000, SDS-PAGE Solanum tuberosum
2.7.7.9 More multimeric forms may exist Solanum tuberosum

Synonyms

EC Number Synonyms Comment Organism
2.7.7.9 UGPase
-
 Solanum tuberosum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.9 37
-
 assay at Solanum tuberosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.9 8.5
-
-
 Solanum tuberosum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.9 7.5 9
-
 Solanum tuberosum