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Literature summary extracted from

  • Sellmann, E.; Schröder, K.L.; Knoblich, I.M.; Westermann, P.
    Purification and characterization of DNA polymerases from Bacillus species (1992), J. Bacteriol., 174, 4350-4355.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.7.7 biotechnology thermostable polymerase used in PCR Thermus thermophilus
2.7.7.7 molecular biology thermostable polymerase used in PCR Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.7 Dideoxynucleoside 5'-triphosphate
-
 [Bacillus] caldotenax
2.7.7.7 Dideoxynucleoside 5'-triphosphate
-
 [Bacillus] caldovelox
2.7.7.7 Dideoxynucleoside 5'-triphosphate
-
 Geobacillus stearothermophilus
2.7.7.7 Dideoxynucleoside 5'-triphosphate
-
 Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.7 K+ stimulates Thermus thermophilus
2.7.7.7 K+ stimulates Geobacillus stearothermophilus
2.7.7.7 K+ stimulates [Bacillus] caldotenax
2.7.7.7 K+ stimulates [Bacillus] caldovelox
2.7.7.7 K+ 5-100 mM Thermus thermophilus
2.7.7.7 K+ stimulates at 100-200 mM Thermus thermophilus
2.7.7.7 K+ stimulates at 100-200 mM Geobacillus stearothermophilus
2.7.7.7 K+ stimulates at 100-200 mM [Bacillus] caldovelox
2.7.7.7 Mg2+ divalent cation required, Mg2+ or Mn2+ Thermus thermophilus
2.7.7.7 Mg2+ divalent cation required, Mg2+ or Mn2+ Geobacillus stearothermophilus
2.7.7.7 Mg2+ divalent cation required, Mg2+ or Mn2+ [Bacillus] caldotenax
2.7.7.7 Mg2+ divalent cation required, Mg2+ or Mn2+ [Bacillus] caldovelox
2.7.7.7 Mg2+ optimal concentration: 5-30 mM Geobacillus stearothermophilus
2.7.7.7 Mg2+ optimal concentration: 5-30 mM [Bacillus] caldotenax
2.7.7.7 Mg2+ optimal concentration: 5-30 mM [Bacillus] caldovelox
2.7.7.7 Mg2+ optimal concentration: 20-30 mM Thermus thermophilus
2.7.7.7 Mn2+ stimulates at 0.4-0.8 mM Thermus thermophilus
2.7.7.7 Mn2+ stimulates at 0.4 mM Thermus thermophilus
2.7.7.7 Mn2+ stimulates at 0.4 mM Geobacillus stearothermophilus
2.7.7.7 Mn2+ stimulates at 0.4 mM [Bacillus] caldovelox
2.7.7.7 Mn2+ divalent cation required, Mn2+ or Mg2+ Thermus thermophilus
2.7.7.7 Mn2+ divalent cation required, Mn2+ or Mg2+ Geobacillus stearothermophilus
2.7.7.7 Mn2+ divalent cation required, Mn2+ or Mg2+ [Bacillus] caldotenax
2.7.7.7 Mn2+ divalent cation required, Mn2+ or Mg2+ [Bacillus] caldovelox

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.7 85000
-
 SDS-PAGE Thermus thermophilus
2.7.7.7 95000
-
 SDS-PAGE Geobacillus stearothermophilus
2.7.7.7 95000
-
 SDS-PAGE [Bacillus] caldovelox

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn Thermus thermophilus
-
 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn Geobacillus stearothermophilus
-
 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn [Bacillus] caldotenax
-
 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn [Bacillus] caldovelox
-
 diphosphate + DNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Geobacillus stearothermophilus
-
-
-
2.7.7.7 Thermus thermophilus
-
-
-
2.7.7.7 [Bacillus] caldotenax
-
-
-
2.7.7.7 [Bacillus] caldovelox
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7
-
 Thermus thermophilus
2.7.7.7
-
 Geobacillus stearothermophilus
2.7.7.7
-
 [Bacillus] caldotenax
2.7.7.7
-
 [Bacillus] caldovelox

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.7 20
-
-
 Thermus thermophilus
2.7.7.7 20.8
-
-
 [Bacillus] caldovelox
2.7.7.7 26.7
-
-
 Geobacillus stearothermophilus
2.7.7.7 33.3
-
-
 [Bacillus] caldotenax

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Thermus thermophilus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Geobacillus stearothermophilus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 [Bacillus] caldotenax diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 [Bacillus] caldovelox diphosphate + DNAn+1
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.7 60 65
-
 Geobacillus stearothermophilus
2.7.7.7 65 70
-
 [Bacillus] caldotenax
2.7.7.7 65 70
-
 [Bacillus] caldovelox
2.7.7.7 70 75
-
 Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.7 80
-
 10 min, inactivation Geobacillus stearothermophilus
2.7.7.7 85
-
 10 min, inactivation [Bacillus] caldotenax
2.7.7.7 85
-
 10 min, inactivation [Bacillus] caldovelox
2.7.7.7 95
-
 10 min, inactivation Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.7 8.8
-
-
 Thermus thermophilus
2.7.7.7 8.8
-
-
 Geobacillus stearothermophilus
2.7.7.7 8.8
-
-
 [Bacillus] caldotenax
2.7.7.7 8.8
-
-
 [Bacillus] caldovelox