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Literature summary extracted from

  • Zhu, W.; Ito, J.
    Purification and characterization of PRD1 DNA polymerase (1994), Biochim. Biophys. Acta, 1219, 267-276.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.7 aphidicolin
-
 Enterobacteria phage PRD1
2.7.7.7 dideoxyguanosine triphosphate
-
 Enterobacteria phage PRD1
2.7.7.7 K+ 50% inhibition at 270 mM Enterobacteria phage PRD1
2.7.7.7 KCl
-
 Enterobacteria phage PRD1
2.7.7.7 N-ethylmaleimide
-
 Enterobacteria phage PRD1
2.7.7.7 NaCl
-
 Enterobacteria phage PRD1
2.7.7.7 NH4Cl
-
 Enterobacteria phage PRD1

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.7 additional information bacteriophage PRD1 enzyme overexpressed in Escherichia coli has a soluble and an insoluble activity indistinguishable by enzymatic properties Enterobacteria phage PRD1
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.7 63000
-
 two-dimensional electrophoresis, second dimension: SDS-PAGE Enterobacteria phage PRD1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn Enterobacteria phage PRD1
-
 diphosphate + DNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Enterobacteria phage PRD1
-
 enzyme overexpressed in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7 enzyme overexpressed in Escherichia coli Enterobacteria phage PRD1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Enterobacteria phage PRD1 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn exonuclease 3'--5' activity Enterobacteria phage PRD1 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn template specificity, enzyme overexpressed in E. coli Enterobacteria phage PRD1 diphosphate + DNAn+1
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.7 2.5 4 deoxynucleoside triphosphate pH 7.6, 30°C Enterobacteria phage PRD1