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Literature summary extracted from

  • Schlüter, A.; Nohlen, M.; Kramer, M.; Defez, R.; Priefer, U.B.
    The Rhizobium leguminosarum bv. viciae glnD gene, encoding a uridylyltransferase/uridylyl-removing enzyme, is expressed in the root nodule but is not essential for nitrogen fixation (2000), Microbiology, 146, 2987-2996.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.59 DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli, recombination of mutant gene into genomic glnD region via double crossover, mutnat VF39-BB3 and VF39-BB1 Rhizobium leguminosarum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.59 additional information mutant construction by transposon Tn5 insertion and by subcloning + double crossover for recombination, phenotype characterization. Mutants are unable to utilize nitrate, essential function of glnD since most mutations close to the 5'-end are lethal Rhizobium leguminosarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.59 UTP + [protein-PII] Rhizobium leguminosarum enzyme is not essential for nitrogen fixation of the host plant diphosphate + uridylyl-[protein-PII]
-
 ?
2.7.7.59 UTP + [protein-PII] Rhizobium leguminosarum the enzyme acts as the primary nitrogen sensor in the nitrogen regulation system diphosphate + uridylyl-[protein-PII]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.59 Rhizobium leguminosarum Q9RAE4 from root nodules of Vicia hirsuta
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2.7.7.59 Rhizobium leguminosarum Q9RAE4 bv. viciae, constitutive gene glnD
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.59 root nodule enzyme expression Rhizobium leguminosarum
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.59 UTP + [protein-PII]
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 Rhizobium leguminosarum diphosphate + uridylyl-[protein-PII]
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 ?
2.7.7.59 UTP + [protein-PII] enzyme is not essential for nitrogen fixation of the host plant Rhizobium leguminosarum diphosphate + uridylyl-[protein-PII]
-
 ?
2.7.7.59 UTP + [protein-PII] the enzyme acts as the primary nitrogen sensor in the nitrogen regulation system Rhizobium leguminosarum diphosphate + uridylyl-[protein-PII]
-
 ?