Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Garcia, E.; Rhee, S.G.
    Cascade control of Escherichia coli glutamine synthetase (1983), J. Biol. Chem., 258, 2246-2253.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.59 gene glnD, overexpression from plasmid in an Escherichia coli strain Escherichia coli

General Stability

EC Number General Stability Organism
2.7.7.59 freezing and thawing of the purified enzyme results in 35-50% loss of activity Escherichia coli
2.7.7.59 purification drastically reduces enzyme stability Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.59 CMP inhibits uridylyl removing activity Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.59 94000
-
 monomeric enzyme form, HPLC gel filtration Escherichia coli
2.7.7.59 186000
-
 dimeric enzyme form, HPLC gel filtration Escherichia coli
2.7.7.59 550000
-
 hexameric form, HPLC gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.59 UTP + [protein-PII] Escherichia coli the enzyme is regulated by Gln and controls the activity of PII signal transduction protein diphosphate + uridylyl-[protein-PII]
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.59 Escherichia coli
-
 gene glnD
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.59 1720fold to homogeneity from recombinant overexpressing strain Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.59 additional information
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.7.59 -70°C, several months without loss of activity Escherichia coli
2.7.7.59 4°C, purified enzyme, in presence of ATP and 0.2 M KCl, 10 h, loss of 15-20% activity compared to storage conditions at -5°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.59 UTP + [protein-PII]
-
 Escherichia coli diphosphate + uridylyl-[protein-PII]
-
 r
2.7.7.59 UTP + [protein-PII] the enzyme is regulated by Gln and controls the activity of PII signal transduction protein Escherichia coli diphosphate + uridylyl-[protein-PII]
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.59 dimer 2 * 94000, the enzyme is detected as monomeric, dimeric and hexameric form, HPLC after equilibration with SDS Escherichia coli
2.7.7.59 hexamer 6 * 94000, the enzyme is detected as monomeric, dimeric and hexameric form, HPLC after equilibration with SDS Escherichia coli
2.7.7.59 monomer 1 * 94000, the enzyme is detected as monomeric, dimeric and hexameric form, HPLC after equilibration with SDS Escherichia coli