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Literature summary extracted from

  • Deutscher, M.P.; Marshall, G.T.; Cudney, H.
    RNase PH: an Escherichia coli phosphate-dependent nuclease distinct from polynucleotide phosphorylase (1988), Proc. Natl. Acad. Sci. USA, 85, 4710-4714.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.56 Mg2+ required Escherichia coli
2.7.7.56 Mg2+ maximal at 3-10 mM Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.56 45000 50000 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.56 tRNAn+1 + phosphate Escherichia coli brings about the final exonucleolytic trimming of the 3'-terminus of tRNA precursors in E. coli by phosphorolysis, producing a mature 3'-terminus on tRNA and nucleoside diphosphate tRNA + a nucleoside diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.56 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.56 poly(A) + phosphate phosphorolysis of poly(A) 15times more rapidly than of tRNA-C-C-A-Cn Escherichia coli ?
-
 r
2.7.7.56 tRNAn+1 + phosphate phosphorolysis of poly(A) 15times more rapidly than of tRNA-C-C-A-Cn Escherichia coli tRNA + a nucleoside diphosphate
-
 r
2.7.7.56 tRNAn+1 + phosphate tRNA-C-C-A-Cn Escherichia coli tRNA + a nucleoside diphosphate
-
 r
2.7.7.56 tRNAn+1 + phosphate brings about the final exonucleolytic trimming of the 3'-terminus of tRNA precursors in E. coli by phosphorolysis, producing a mature 3'-terminus on tRNA and nucleoside diphosphate Escherichia coli tRNA + a nucleoside diphosphate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.56 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.56 8
-
-
 Escherichia coli