BRENDA - Enzyme Database

Anabaena flos-aquae and other cyanobacteria possess diadenosine 5',5''-P1,P4-tetraphosphate (Ap4A) phosphorylase activity

McLennan, A.G.; Mayers, E.; Adams, D.G.; Biochem. J. 320, 795-800 (1996)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.53
0.006
-
P1,P4-bis(5'-adenosyl) tetraphosphate
pH 9.5
Dolichospermum flos-aquae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.53
Ca2+
activates
Dolichospermum flos-aquae
2.7.7.53
Co2+
activates
Dolichospermum flos-aquae
2.7.7.53
Mg2+
required, most effective divalent cation
Dolichospermum flos-aquae
2.7.7.53
Mn2+
activates
Dolichospermum flos-aquae
2.7.7.53
Zn2+
slight activation
Dolichospermum flos-aquae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.53
32000
-
gel filtration
Dolichospermum flos-aquae
2.7.7.53
32000
-
gel filtration
Trichormus variabilis
2.7.7.53
39000
-
gel filtration
Synechococcus sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.53
Dolichospermum flos-aquae
-
-
-
2.7.7.53
Synechococcus sp.
-
-
-
2.7.7.53
Trichormus variabilis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.7.53
partial, 206fold
Dolichospermum flos-aquae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.7.53
0.037
-
partially purified enzyme
Dolichospermum flos-aquae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.53
additional information
P1,P6-bis(5'-adenosyl) hexaphosphate is a very poor substrate
643515
Dolichospermum flos-aquae
?
-
-
-
-
2.7.7.53
additional information
no activity with sulfate
643515
Dolichospermum flos-aquae
?
-
-
-
-
2.7.7.53
P1,P2-bis(5'-adenosyl) diphosphate + phosphate
no activity
643515
Dolichospermum flos-aquae
?
-
-
-
-
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
-
643515
Dolichospermum flos-aquae
ADP
-
643515
Dolichospermum flos-aquae
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate
182% activity compared to phosphate
643515
Dolichospermum flos-aquae
?
-
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + chromate
poor substrate
643515
Dolichospermum flos-aquae
?
-
-
-
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + molybdate
poor substrate
643515
Dolichospermum flos-aquae
?
-
-
-
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643515
Dolichospermum flos-aquae
ADP + ATP
-
643515
Dolichospermum flos-aquae
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643515
Synechococcus sp.
ADP + ATP
-
643515
Synechococcus sp.
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643515
Trichormus variabilis
ADP + ATP
-
643515
Trichormus variabilis
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + tungstate
no activity
643515
Dolichospermum flos-aquae
?
-
-
-
-
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + vanadate
225% activity compared to phosphate
643515
Dolichospermum flos-aquae
?
-
-
-
r
2.7.7.53
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
-
643515
Dolichospermum flos-aquae
ADP + p4A
-
643515
Dolichospermum flos-aquae
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.53
9
-
-
Dolichospermum flos-aquae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.53
0.006
-
P1,P4-bis(5'-adenosyl) tetraphosphate
pH 9.5
Dolichospermum flos-aquae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.53
Ca2+
activates
Dolichospermum flos-aquae
2.7.7.53
Co2+
activates
Dolichospermum flos-aquae
2.7.7.53
Mg2+
required, most effective divalent cation
Dolichospermum flos-aquae
2.7.7.53
Mn2+
activates
Dolichospermum flos-aquae
2.7.7.53
Zn2+
slight activation
Dolichospermum flos-aquae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.53
32000
-
gel filtration
Dolichospermum flos-aquae
2.7.7.53
32000
-
gel filtration
Trichormus variabilis
2.7.7.53
39000
-
gel filtration
Synechococcus sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.7.53
partial, 206fold
Dolichospermum flos-aquae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.7.53
0.037
-
partially purified enzyme
Dolichospermum flos-aquae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.53
additional information
P1,P6-bis(5'-adenosyl) hexaphosphate is a very poor substrate
643515
Dolichospermum flos-aquae
?
-
-
-
-
2.7.7.53
additional information
no activity with sulfate
643515
Dolichospermum flos-aquae
?
-
-
-
-
2.7.7.53
P1,P2-bis(5'-adenosyl) diphosphate + phosphate
no activity
643515
Dolichospermum flos-aquae
?
-
-
-
-
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
-
643515
Dolichospermum flos-aquae
ADP
-
643515
Dolichospermum flos-aquae
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate
182% activity compared to phosphate
643515
Dolichospermum flos-aquae
?
-
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + chromate
poor substrate
643515
Dolichospermum flos-aquae
?
-
-
-
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + molybdate
poor substrate
643515
Dolichospermum flos-aquae
?
-
-
-
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643515
Dolichospermum flos-aquae
ADP + ATP
-
643515
Dolichospermum flos-aquae
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643515
Synechococcus sp.
ADP + ATP
-
643515
Synechococcus sp.
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643515
Trichormus variabilis
ADP + ATP
-
643515
Trichormus variabilis
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + tungstate
no activity
643515
Dolichospermum flos-aquae
?
-
-
-
-
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + vanadate
225% activity compared to phosphate
643515
Dolichospermum flos-aquae
?
-
-
-
r
2.7.7.53
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
-
643515
Dolichospermum flos-aquae
ADP + p4A
-
643515
Dolichospermum flos-aquae
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.53
9
-
-
Dolichospermum flos-aquae