BRENDA - Enzyme Database

Diadenosine 5,5-P1, P4-tetraphosphate alpha, beta-phosphorylase from yeast supports nucleoside diphosphate-phosphate exchange

Guranowski, A.; Blanquet, S.; J. Biol. Chem. 261, 5943-5946 (1986)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.7.7.53
dithioerythritol stabilizes
Saccharomyces cerevisiae
2.7.7.53
glycerol, 10%, stabilizes
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.53
AMP
ADP/phosphate-exchange
Saccharomyces cerevisiae
2.7.7.53
Ap3A
ADP/phosphate-exchange
Saccharomyces cerevisiae
2.7.7.53
Ca2+
weak, NDP-arsenolysis or NDP/phosphate-exchange reaction
Saccharomyces cerevisiae
2.7.7.53
Cd2+
only NDP-arsenolysis or NDP/phosphate-exchange reaction
Saccharomyces cerevisiae
2.7.7.53
Co2+
weak, NDP-arsenolysis or NDP/phosphate-exchange reaction
Saccharomyces cerevisiae
2.7.7.53
Mg2+
only NDP-arsenolysis or NDP/phosphate-exchange reaction
Saccharomyces cerevisiae
2.7.7.53
Mn2+
weak, NDP-arsenolysis or NDP/phosphate-exchange reaction
Saccharomyces cerevisiae
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate
ADP/phosphate-exchange
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.53
additional information
-
additional information
exchange reaction with ADP and analogues
Saccharomyces cerevisiae
2.7.7.53
0.7
-
ADP
ADP/phosphate-exchange, pH 7.0, 37°C
Saccharomyces cerevisiae
2.7.7.53
0.7
-
GDP
ADP/phosphate-exchange, pH 7.0, 37°C
Saccharomyces cerevisiae
2.7.7.53
2
-
phosphate
ADP/phosphate-exchange, pH 7.0, 37°C
Saccharomyces cerevisiae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.53
Mn2+
not NDP-arsenolysis or NDP/phosphate-exchange reaction
Saccharomyces cerevisiae
2.7.7.53
additional information
no divalent metal cation requirement for NDP-arsenolysis or NDP/phosphate-exchange reaction
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.53
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.53
additional information
broad substrate specificity
643510
Saccharomyces cerevisiae
?
-
-
-
-
2.7.7.53
NDP + arsenate
-
643510
Saccharomyces cerevisiae
NMP + phosphate + ?
-
643510
Saccharomyces cerevisiae
?
2.7.7.53
NDP + phosphate
exchange reaction between beta-phosphate of NDP and phosphate from the medium
643510
Saccharomyces cerevisiae
phosphate + NDP
-
643510
Saccharomyces cerevisiae
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643510
Saccharomyces cerevisiae
ADP + ATP
-
643510
Saccharomyces cerevisiae
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.7.53
37
-
assay at
Saccharomyces cerevisiae
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.7.53
96
-
ADP
ADP/phosphate-exchange, pH 7.0, 37°C
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.53
additional information
-
pH-dependence of NDP/phosphate-exchange reaction
Saccharomyces cerevisiae
2.7.7.53
6.5
-
UDP + phosphate, NDP/phosphate-exchange, ADP, GDP or CDP + arsenate, NDP-arsenolysis
Saccharomyces cerevisiae
2.7.7.53
7
-
ADP or CDP + phosphate, NDP/phosphate-exchange
Saccharomyces cerevisiae
2.7.7.53
8
-
GDP + phosphate, NDP/phosphate-exchange
Saccharomyces cerevisiae
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.7.53
dithioerythritol stabilizes
Saccharomyces cerevisiae
2.7.7.53
glycerol, 10%, stabilizes
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.53
AMP
ADP/phosphate-exchange
Saccharomyces cerevisiae
2.7.7.53
Ap3A
ADP/phosphate-exchange
Saccharomyces cerevisiae
2.7.7.53
Ca2+
weak, NDP-arsenolysis or NDP/phosphate-exchange reaction
Saccharomyces cerevisiae
2.7.7.53
Cd2+
only NDP-arsenolysis or NDP/phosphate-exchange reaction
Saccharomyces cerevisiae
2.7.7.53
Co2+
weak, NDP-arsenolysis or NDP/phosphate-exchange reaction
Saccharomyces cerevisiae
2.7.7.53
Mg2+
only NDP-arsenolysis or NDP/phosphate-exchange reaction
Saccharomyces cerevisiae
2.7.7.53
Mn2+
weak, NDP-arsenolysis or NDP/phosphate-exchange reaction
Saccharomyces cerevisiae
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate
ADP/phosphate-exchange
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.53
additional information
-
additional information
exchange reaction with ADP and analogues
Saccharomyces cerevisiae
2.7.7.53
0.7
-
ADP
ADP/phosphate-exchange, pH 7.0, 37°C
Saccharomyces cerevisiae
2.7.7.53
0.7
-
GDP
ADP/phosphate-exchange, pH 7.0, 37°C
Saccharomyces cerevisiae
2.7.7.53
2
-
phosphate
ADP/phosphate-exchange, pH 7.0, 37°C
Saccharomyces cerevisiae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.53
Mn2+
not NDP-arsenolysis or NDP/phosphate-exchange reaction
Saccharomyces cerevisiae
2.7.7.53
additional information
no divalent metal cation requirement for NDP-arsenolysis or NDP/phosphate-exchange reaction
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.53
additional information
broad substrate specificity
643510
Saccharomyces cerevisiae
?
-
-
-
-
2.7.7.53
NDP + arsenate
-
643510
Saccharomyces cerevisiae
NMP + phosphate + ?
-
643510
Saccharomyces cerevisiae
?
2.7.7.53
NDP + phosphate
exchange reaction between beta-phosphate of NDP and phosphate from the medium
643510
Saccharomyces cerevisiae
phosphate + NDP
-
643510
Saccharomyces cerevisiae
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643510
Saccharomyces cerevisiae
ADP + ATP
-
643510
Saccharomyces cerevisiae
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.7.53
37
-
assay at
Saccharomyces cerevisiae
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.7.53
96
-
ADP
ADP/phosphate-exchange, pH 7.0, 37°C
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.53
additional information
-
pH-dependence of NDP/phosphate-exchange reaction
Saccharomyces cerevisiae
2.7.7.53
6.5
-
UDP + phosphate, NDP/phosphate-exchange, ADP, GDP or CDP + arsenate, NDP-arsenolysis
Saccharomyces cerevisiae
2.7.7.53
7
-
ADP or CDP + phosphate, NDP/phosphate-exchange
Saccharomyces cerevisiae
2.7.7.53
8
-
GDP + phosphate, NDP/phosphate-exchange
Saccharomyces cerevisiae