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Literature summary extracted from

  • Shuman, S.; Hurwitz, J.
    Capping enzyme (1982), The Enzymes, 3rd. Ed. (Boyer, P. D. , ed. ), 15, 245-265.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.50 S-adenosylmethionine stimulates up to 2fold Vaccinia virus

Application

EC Number Application Comment Organism
2.7.7.50 analysis valuable in a variety of analytical studies of RNA Vaccinia virus
2.7.7.50 biotechnology enzyme can be used as a tool for specific 5'-end-labeling of mRNA Vaccinia virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.50 diphosphate
-
 Homo sapiens
2.7.7.50 diphosphate strong product inhibition Vaccinia virus
2.7.7.50 additional information no inhibition by S-adenosylmethionine and S-adenosylhomocysteine Homo sapiens
2.7.7.50 additional information
-
 Vaccinia virus
2.7.7.50 N-ethylmaleimide
-
 Homo sapiens
2.7.7.50 NaCl 90% inhibition at 0.3 M Vaccinia virus
2.7.7.50 phosphate above 20 mM Vaccinia virus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.50 0.000285
-
 pp(5')ApGp pH 7.8, 37°C Homo sapiens
2.7.7.50 0.015
-
 GTP pH 7.8, 37°C Vaccinia virus
2.7.7.50 0.019
-
 diphosphate terminated poly(A) with an average chain length of 2000 nucleotides
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.50 nucleus
-
 Homo sapiens 5634
-
2.7.7.50 nucleus
-
 Rattus norvegicus 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.50 Mg2+ required Homo sapiens
2.7.7.50 Mg2+ required Vaccinia virus
2.7.7.50 Mg2+ can partially replace Mn2+ in activation Homo sapiens
2.7.7.50 Mg2+ can partially replace Mn2+ in activation Rattus norvegicus
2.7.7.50 Mg2+ 25% of the activity with Mn2+ Homo sapiens
2.7.7.50 Mn2+ required Homo sapiens
2.7.7.50 Mn2+ required Rattus norvegicus
2.7.7.50 Mn2+ can partially replace Mg2+ in activation Homo sapiens
2.7.7.50 Mn2+ can partially replace Mg2+ in activation Rattus norvegicus
2.7.7.50 Mn2+ can partially replace Mg2+ in activation Vaccinia virus
2.7.7.50 Mn2+ maximal activity at 2 mM Homo sapiens
2.7.7.50 additional information no activation by Ca2+ or Zn2+ Vaccinia virus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.50 26400
-
 x * 95000, subunit containing the active site + x * 26400, transguanylyltransferase subunit, SDS-PAGE Vaccinia virus
2.7.7.50 48500
-
 sucrose density gradient sedimentation Homo sapiens
2.7.7.50 65000
-
 gel filtration Rattus norvegicus
2.7.7.50 95000
-
 x * 95000, subunit containing the active site + x * 26400, transguanylyltransferase subunit, SDS-PAGE Vaccinia virus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.50 GTP + pp(5')RNA Homo sapiens
-
 G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue ir
2.7.7.50 GTP + pp(5')RNA Rattus norvegicus
-
 G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r
2.7.7.50 GTP + pp(5')RNA Vaccinia virus
-
 G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r
2.7.7.50 GTP + ppp(5')RNA Homo sapiens
-
 G(5')pppp(5')RNA + diphosphate guanosine residue linked 5' through four phosphates to the 5' position of the terminal residue ir
2.7.7.50 GTP + ppp(5')RNA Rattus norvegicus
-
 G(5')pppp(5')RNA + diphosphate guanosine residue linked 5' through four phosphates to the 5' position of the terminal residue r
2.7.7.50 GTP + ppp(5')RNA Vaccinia virus
-
 G(5')pppp(5')RNA + diphosphate guanosine residue linked 5' through four phosphates to the 5' position of the terminal residue r
2.7.7.50 additional information Homo sapiens mRNA capping enzyme ?
-
 ?
2.7.7.50 additional information Rattus norvegicus mRNA capping enzyme ?
-
 ?
2.7.7.50 additional information Vaccinia virus mRNA capping enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.50 Homo sapiens
-
 HeLa cells
-
2.7.7.50 Rattus norvegicus
-
-
-
2.7.7.50 Vaccinia virus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.50
-
 Vaccinia virus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.50 GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA mechanism of capping Homo sapiens
a
2.7.7.50 GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA mechanism of capping Rattus norvegicus
a
2.7.7.50 GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA mechanism of capping Vaccinia virus
a
2.7.7.50 GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA mRNA containing a guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue Homo sapiens
a
2.7.7.50 GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA mRNA containing a guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue Rattus norvegicus
a
2.7.7.50 GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA mRNA containing a guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue Vaccinia virus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.50 HeLa cell
-
 Homo sapiens
-
2.7.7.50 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.50 dGTP + pp(5')RNA
-
 Vaccinia virus dG(5')ppp(5')RNA + diphosphate deoxyguanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r
2.7.7.50 dGTP + pp(5')RNA no activity Homo sapiens dG(5')ppp(5')RNA + diphosphate
-
 ?
2.7.7.50 GTP + diphosphate terminated poly(A) with an average chain length of 2000 nucleotides
-
 Homo sapiens ?
-
 ?
2.7.7.50 GTP + pp(5')ApGp
-
 Homo sapiens G(5')ppp(5')ApGp + diphosphate
-
 ir
2.7.7.50 GTP + pp(5')GpCpC
-
 Rattus norvegicus G(5')ppp(5')GpCpC + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r
2.7.7.50 GTP + pp(5')RNA
-
 Homo sapiens G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue ir
2.7.7.50 GTP + pp(5')RNA
-
 Rattus norvegicus G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r
2.7.7.50 GTP + pp(5')RNA
-
 Vaccinia virus G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r
2.7.7.50 GTP + pp(5')RNA specific for GTP Vaccinia virus G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r
2.7.7.50 GTP + pp(5')RNA no apparent base specificity for the penultimate nucleotide, a variety of synthetic homoribopolymers and naturally occuring mRNAs are effective substrates, vaccinia virus Vaccinia virus G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r
2.7.7.50 GTP + pp(5')RNA the acceptor is diphosphate terminated poly(A) Homo sapiens G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue ir
2.7.7.50 GTP + pp(5')RNA the acceptor is diphosphate terminated poly(A) Homo sapiens G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r
2.7.7.50 GTP + pp(5')RNA the acceptor is diphosphate terminated poly(A) Vaccinia virus G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r
2.7.7.50 GTP + ppApG
-
 Vaccinia virus G(5')ppp(5')ApG + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r
2.7.7.50 GTP + ppGpC
-
 Homo sapiens G(5')ppp(5')GpC + diphosphate
-
 ir
2.7.7.50 GTP + ppGpC
-
 Vaccinia virus G(5')ppp(5')GpC + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue ir
2.7.7.50 GTP + ppp(5')ApG
-
 Homo sapiens G(5')pppp(5')ApG + diphosphate
-
 ir
2.7.7.50 GTP + ppp(5')ApG
-
 Vaccinia virus G(5')pppp(5')ApG + diphosphate guanosine residue linked 5' through four phosphates to the 5' position of the terminal residue r
2.7.7.50 GTP + ppp(5')RNA
-
 Homo sapiens G(5')pppp(5')RNA + diphosphate guanosine residue linked 5' through four phosphates to the 5' position of the terminal residue ir
2.7.7.50 GTP + ppp(5')RNA
-
 Rattus norvegicus G(5')pppp(5')RNA + diphosphate guanosine residue linked 5' through four phosphates to the 5' position of the terminal residue r
2.7.7.50 GTP + ppp(5')RNA
-
 Vaccinia virus G(5')pppp(5')RNA + diphosphate guanosine residue linked 5' through four phosphates to the 5' position of the terminal residue r
2.7.7.50 GTP + pppGpCpC
-
 Rattus norvegicus G(5')pppp(5')GpCpC + diphosphate guanosine residue linked 5' through four phosphates to the 5' position of the terminal residue r
2.7.7.50 GTPgammaS + pp(5')RNA
-
 Vaccinia virus ?
-
 r
2.7.7.50 ITP + pp(5')RNA
-
 Homo sapiens I(5')ppp(5')RNA + diphosphate inosine residue linked 5' through three phosphates to the 5' position of the terminal residue ir
2.7.7.50 additional information activities associated with vaccinia capping enzyme complex: 1. GTP-RNA guanylyltransferase, 2. RNA (guanine-7)-methyltransferase, 3. RNA triphosphatase, 4. GTP-diphosphate exchange, 5. nucleoside triphosphate phosphorylase Vaccinia virus ?
-
 ?
2.7.7.50 additional information no activity with GMP Vaccinia virus ?
-
 ?
2.7.7.50 additional information no acceptor: RNA with 5'-hydroxyl terminus Vaccinia virus ?
-
 ?
2.7.7.50 additional information no activity with GDP Homo sapiens ?
-
 ?
2.7.7.50 additional information no activity with GDP Vaccinia virus ?
-
 ?
2.7.7.50 additional information no donor: 7-methylGTP Homo sapiens ?
-
 ?
2.7.7.50 additional information no donor: 7-methylGTP Vaccinia virus ?
-
 ?
2.7.7.50 additional information no acceptor: RNA with a single 5'-terminal phosphate Vaccinia virus ?
-
 ?
2.7.7.50 additional information enzyme also catalyzes GTP-diphosphate exchange Homo sapiens ?
-
 ?
2.7.7.50 additional information enzyme also catalyzes GTP-diphosphate exchange Rattus norvegicus ?
-
 ?
2.7.7.50 additional information enzyme also catalyzes GTP-diphosphate exchange Vaccinia virus ?
-
 ?
2.7.7.50 additional information no activity with ATP, CTP, UTP Homo sapiens ?
-
 ?
2.7.7.50 additional information no activity with ATP, CTP, UTP Vaccinia virus ?
-
 ?
2.7.7.50 additional information mRNA capping enzyme Homo sapiens ?
-
 ?
2.7.7.50 additional information mRNA capping enzyme Rattus norvegicus ?
-
 ?
2.7.7.50 additional information mRNA capping enzyme Vaccinia virus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.50 ? x * 95000, subunit containing the active site + x * 26400, transguanylyltransferase subunit, SDS-PAGE Vaccinia virus

Synonyms

EC Number Synonyms Comment Organism
2.7.7.50 More activities associated with vaccinia capping enzyme complex: 1. GTP-RNA guanylyltransferase, 2. RNA (guanine-7)-methyltransferase, 3. RNA triphosphatase, 4. GTP-diphosphate exchange, 5. nucleoside triphosphate phosphorylase Vaccinia virus
2.7.7.50 mRNA capping enzyme activities associated with vaccinia capping enzyme complex: 1. GTP-RNA guanylyltransferase, 2. RNA (guanine-7)-methyltransferase, 3. RNA triphosphatase, 4. GTP-PPi exchange, 5. nucleoside triphosphate phosphorylase Vaccinia virus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.50 37
-
 assay at Homo sapiens
2.7.7.50 37
-
 assay at Rattus norvegicus
2.7.7.50 37
-
 assay at Vaccinia virus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.50 50
-
 5 min, complete inactivation Vaccinia virus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.50 7.5
-
-
 Homo sapiens
2.7.7.50 7.8
-
 Tris-HCl buffer Vaccinia virus
2.7.7.50 7.8
-
 assay at Homo sapiens
2.7.7.50 7.8
-
 assay at Rattus norvegicus
2.7.7.50 7.8
-
 assay at Vaccinia virus