Literature summary extracted from
Leis, J.; Hurwitz, J.
RNA-dependent DNA polymerase from avian myeloblastosis virus (1974), Methods Enzymol., 29E, 143-150.
No PubMed abstract available
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
2.7.7.49 |
dithiothreitol |
sulfhydryl reagents required for optimal activity: dithiothreitol or 2-mercaptoethanol |
Avian myeloblastosis virus |
|
General Stability
EC Number |
General Stability |
Organism |
---|
2.7.7.49 |
presence of 0.2 M ammonium sulfate helps to stabilize the enzyme |
Avian myeloblastosis virus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.7.7.49 |
KCl |
80 mM, 50% inhibition |
Avian myeloblastosis virus |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
2.7.7.49 |
additional information |
- |
additional information |
Km-value for deoxynucleoside triphosphates is 0.01-0.03 mM |
Avian myeloblastosis virus |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.7.7.49 |
K+ |
5 mM, stimulates rate of DNA synthesis by 20% |
Avian myeloblastosis virus |
|
2.7.7.49 |
Mg2+ |
requires Mg2+ for activity with DNA or AMV RMA. Optimal activity at 10 mM |
Avian myeloblastosis virus |
|
2.7.7.49 |
Mn2+ |
can partially replace Mg2+ |
Avian myeloblastosis virus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.7.49 |
Avian myeloblastosis virus |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.7.49 |
- |
Avian myeloblastosis virus |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
2.7.7.49 |
0.000104 |
- |
above |
Avian myeloblastosis virus |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
2.7.7.49 |
-20°C, 50% glycerol, 2 mM dithioerythritol, less than 20% loss of activity after 2 months |
Avian myeloblastosis virus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.7.49 |
deoxynucleoside triphosphate + DNAn |
the polymerase requires a primer strand with free 3'-hydroxyl group and a template strand to direct DNA synthesis |
Avian myeloblastosis virus |
diphosphate + DNAn+1 |
- |
? |
|
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
2.7.7.49 |
48 |
- |
at high glycerol concehntrations, 2.5 min, 50% loss of activity |
Avian myeloblastosis virus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
2.7.7.49 |
8.2 |
- |
- |
Avian myeloblastosis virus |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
2.7.7.49 |
7.2 |
9.8 |
about 50% of maximal activity at pH 7.2 and pH 9.8 |
Avian myeloblastosis virus |