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Literature summary extracted from

  • Inouye, S.; Inouye, M.
    Bacterial reverse transcriptase (1993), Cold Spring Harbor Monogr. Ser., 23, 391-410.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.49 65000
-
 x * 65000, SDS-PAGE Escherichia coli
2.7.7.49 67227
-
 x * 67227, calculation from nucleotide sequence Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.49 Escherichia coli
-
-
-
2.7.7.49 Myxococcus xanthus
-
-
-
2.7.7.49 Stigmatella aurantiaca
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.49 it is important to purify RT-Ec67 from an Escherichia coli strain defective in DNA polymerase I, because this enzyme can utilize an RNA template to synthesize DNA Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.49 deoxynucleoside triphosphate + DNAn
-
 Myxococcus xanthus diphosphate + DNAn+1
-
 ?
2.7.7.49 deoxynucleoside triphosphate + DNAn
-
 Stigmatella aurantiaca diphosphate + DNAn+1
-
 ?
2.7.7.49 deoxynucleoside triphosphate + DNAn the purified enzyme can synthesize DNA using RNA as a template and a synthetic oligodeoxynucleotide as a primer: cDNA can be synthesized using the Escherichi coli 5S RNA as template and a 15-base synthetic oligonucleotide complementary to the 3'-end of the 5S RNA as a primer. The enzyme can also produce a full-length cDNA using a 50-base synthetic DNA as a template and a synthetic oligonucleotide complementary to the 3'-end of the template as a primer Escherichia coli diphosphate + DNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.49 ? x * 65000, SDS-PAGE Escherichia coli
2.7.7.49 ? x * 67227, calculation from nucleotide sequence Escherichia coli