Literature summary extracted from
Plotch, S.J.; Palant, O.; Gluzman, Y.
Purification and properties of Poliovirus RNA polymerase expressed in Escherichia coli (1989), J. Virol., 63, 216-225.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.7.48 |
expression in Escherichia coli, a mutant is generated as a result of the deletion of a trinucleotide in the N-terminal portion of the coding region, pT7-POL(TRP-). The protein expressed from this mutant lacks a tryptophan residue normally present at the fifth amino acid from the N-terminal glycine. This protein has no detectable enzymatic activity. Mutant pT7-POL(AvII), which lacks the C-terminal 53 amino acids of the normal protein is also inactive |
Enterovirus C |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.7.7.48 |
additional information |
a mutant is generated as a result of the deletion of a trinucleotide in the N-terminal portion of the coding region, pT7-POL(TRP-). The protein expressed from this mutant lacks a tryptophan residue normally present at the fifth amino acid from the N-terminal glycine. This protein has no detectable enzymatic activity. Mutant pT7-POL(AvII), which lacks the C-terminal 53 amino acids of the normal protein is also inactive |
Enterovirus C |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.7.48 |
Enterovirus C |
- |
expressed in Escherichia coli |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.7.48 |
- |
Enterovirus C |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.7.48 |
nucleoside triphosphate + RNAn |
poly(A)-dependent oligo(U)-primed poly(U) polymerase activity. In the presence of an oligo(U) primer, the enzyme catalyzes the synthesis of a full-length copy of either poliovirus or globin RNA templates. In the absence of added primer, RNA products up to twice the length of the template are synthesized |
Enterovirus C |
diphosphate + RNAn+1 |
- |
? |
|